1hch: Difference between revisions
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[[Image:1hch.jpg|left|200px]] | [[Image:1hch.jpg|left|200px]] | ||
'''STRUCTURE OF HORSERADISH PEROXIDASE C1A COMPOUND I''' | {{Structure | ||
|PDB= 1hch |SIZE=350|CAPTION= <scene name='initialview01'>1hch</scene>, resolution 1.57Å | |||
|SITE= <scene name='pdbsite=CAA:Heme+Binding+Site'>CAA</scene> | |||
|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene> and <scene name='pdbligand=O:OXYGEN ATOM'>O</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Peroxidase Peroxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.11.1.7 1.11.1.7] | |||
|GENE= | |||
}} | |||
'''STRUCTURE OF HORSERADISH PEROXIDASE C1A COMPOUND I''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1HCH is a [ | 1HCH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Armoracia_rusticana Armoracia rusticana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HCH OCA]. | ||
==Reference== | ==Reference== | ||
The catalytic pathway of horseradish peroxidase at high resolution., Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J, Nature. 2002 May 23;417(6887):463-8. PMID:[http:// | The catalytic pathway of horseradish peroxidase at high resolution., Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J, Nature. 2002 May 23;417(6887):463-8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12024218 12024218] | ||
[[Category: Armoracia rusticana]] | [[Category: Armoracia rusticana]] | ||
[[Category: Peroxidase]] | [[Category: Peroxidase]] | ||
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[[Category: peroxidase]] | [[Category: peroxidase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:35:14 2008'' | ||
Revision as of 09:35, 20 March 2008
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| 1hch, resolution 1.57Å | |||||||||||||
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| Sites: | CAA | ||||||||||||
| Ligands: | ACT, CA, HEM and O | ||||||||||||
| Activity: | Peroxidase, with EC number 1.11.1.7 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
STRUCTURE OF HORSERADISH PEROXIDASE C1A COMPOUND I
Overview
A molecular description of oxygen and peroxide activation in biological systems is difficult, because electrons liberated during X-ray data collection reduce the active centres of redox enzymes catalysing these reactions. Here we describe an effective strategy to obtain crystal structures for high-valency redox intermediates and present a three-dimensional movie of the X-ray-driven catalytic reduction of a bound dioxygen species in horseradish peroxidase (HRP). We also describe separate experiments in which high-resolution structures could be obtained for all five oxidation states of HRP, showing such structures with preserved redox states for the first time.
About this Structure
1HCH is a Single protein structure of sequence from Armoracia rusticana. Full crystallographic information is available from OCA.
Reference
The catalytic pathway of horseradish peroxidase at high resolution., Berglund GI, Carlsson GH, Smith AT, Szoke H, Henriksen A, Hajdu J, Nature. 2002 May 23;417(6887):463-8. PMID:12024218
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