1hjr: Difference between revisions
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'''ATOMIC STRUCTURE OF THE RUVC RESOLVASE: A HOLLIDAY JUNCTION-SPECIFIC ENDONUCLEASE FROM E. COLI''' | {{Structure | ||
|PDB= 1hjr |SIZE=350|CAPTION= <scene name='initialview01'>1hjr</scene>, resolution 2.5Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Crossover_junction_endodeoxyribonuclease Crossover junction endodeoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.22.4 3.1.22.4] | |||
|GENE= | |||
}} | |||
'''ATOMIC STRUCTURE OF THE RUVC RESOLVASE: A HOLLIDAY JUNCTION-SPECIFIC ENDONUCLEASE FROM E. COLI''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1HJR is a [ | 1HJR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJR OCA]. | ||
==Reference== | ==Reference== | ||
Atomic structure of the RuvC resolvase: a holliday junction-specific endonuclease from E. coli., Ariyoshi M, Vassylyev DG, Iwasaki H, Nakamura H, Shinagawa H, Morikawa K, Cell. 1994 Sep 23;78(6):1063-72. PMID:[http:// | Atomic structure of the RuvC resolvase: a holliday junction-specific endonuclease from E. coli., Ariyoshi M, Vassylyev DG, Iwasaki H, Nakamura H, Shinagawa H, Morikawa K, Cell. 1994 Sep 23;78(6):1063-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/7923356 7923356] | ||
[[Category: Crossover junction endodeoxyribonuclease]] | [[Category: Crossover junction endodeoxyribonuclease]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: site-specific recombinase]] | [[Category: site-specific recombinase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:37:58 2008'' | ||
Revision as of 09:37, 20 March 2008
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| 1hjr, resolution 2.5Å | |||||||||||||
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| Activity: | Crossover junction endodeoxyribonuclease, with EC number 3.1.22.4 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
ATOMIC STRUCTURE OF THE RUVC RESOLVASE: A HOLLIDAY JUNCTION-SPECIFIC ENDONUCLEASE FROM E. COLI
Overview
The crystal structure of the RuvC protein, a Holliday junction resolvase from E. coli, has been determined at 2.5 A resolution. The enzyme forms a dimer of 19 kDa subunits related by a dyad axis. Together with results from extensive mutational analyses, the refined structure reveals that the catalytic center, comprising four acidic residues, lies at the bottom of a cleft that nicely fits a DNA duplex. The structural features of the dimer, with a 30 A spacing between the two catalytic centers, provide a substantially defined image of the Holliday junction architecture. The folding topology in the vicinity of the catalytic site exhibits a striking similarity to that of RNAase H1 from E. coli.
About this Structure
1HJR is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Atomic structure of the RuvC resolvase: a holliday junction-specific endonuclease from E. coli., Ariyoshi M, Vassylyev DG, Iwasaki H, Nakamura H, Shinagawa H, Morikawa K, Cell. 1994 Sep 23;78(6):1063-72. PMID:7923356
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