1hww: Difference between revisions
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[[Image:1hww.gif|left|200px]] | [[Image:1hww.gif|left|200px]] | ||
'''GOLGI ALPHA-MANNOSIDASE II IN COMPLEX WITH SWAINSONINE''' | {{Structure | ||
|PDB= 1hww |SIZE=350|CAPTION= <scene name='initialview01'>1hww</scene>, resolution 1.87Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=SWA:1S-8AB-OCTAHYDRO-INDOLIZIDINE-1A,2A,8B-TRIOL'>SWA</scene> and <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Mannosyl-oligosaccharide_1,3-1,6-alpha-mannosidase Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.114 3.2.1.114] | |||
|GENE= | |||
}} | |||
'''GOLGI ALPHA-MANNOSIDASE II IN COMPLEX WITH SWAINSONINE''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1HWW is a [ | 1HWW is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HWW OCA]. | ||
==Reference== | ==Reference== | ||
Structure of Golgi alpha-mannosidase II: a target for inhibition of growth and metastasis of cancer cells., van den Elsen JM, Kuntz DA, Rose DR, EMBO J. 2001 Jun 15;20(12):3008-17. PMID:[http:// | Structure of Golgi alpha-mannosidase II: a target for inhibition of growth and metastasis of cancer cells., van den Elsen JM, Kuntz DA, Rose DR, EMBO J. 2001 Jun 15;20(12):3008-17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11406577 11406577] | ||
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase]] | [[Category: Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase]] | ||
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[[Category: SWA]] | [[Category: SWA]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: 2 c-terminal beta | [[Category: 2 c-terminal beta barrel]] | ||
[[Category: n-terminal alpha-beta domain]] | [[Category: n-terminal alpha-beta domain]] | ||
[[Category: three helix bundle]] | [[Category: three helix bundle]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:42:32 2008'' | ||
Revision as of 09:42, 20 March 2008
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| 1hww, resolution 1.87Å | |||||||||||||
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| Ligands: | NAG, ZN, SWA and MRD | ||||||||||||
| Activity: | Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase, with EC number 3.2.1.114 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
GOLGI ALPHA-MANNOSIDASE II IN COMPLEX WITH SWAINSONINE
Overview
Golgi alpha-mannosidase II, a key enzyme in N-glycan processing, is a target in the development of anti- cancer therapies. The crystal structure of Drosophila Golgi alpha-mannosidase II in the absence and presence of the anti-cancer agent swainsonine and the inhibitor deoxymannojirimycin reveals a novel protein fold with an active site zinc intricately involved both in the substrate specificity of the enzyme and directly in the catalytic mechanism. Identification of a putative GlcNAc binding pocket in the vicinity of the active site cavity provides a model for the binding of the GlcNAcMan(5)GlcNAc(2) substrate and the consecutive hydrolysis of the alpha1,6- and alpha1,3-linked mannose residues. The enzyme-inhibitor interactions observed provide insight into the catalytic mechanism, opening the door to the design of novel inhibitors of alpha-mannosidase II.
About this Structure
1HWW is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Structure of Golgi alpha-mannosidase II: a target for inhibition of growth and metastasis of cancer cells., van den Elsen JM, Kuntz DA, Rose DR, EMBO J. 2001 Jun 15;20(12):3008-17. PMID:11406577
Page seeded by OCA on Thu Mar 20 11:42:32 2008
