1i9f: Difference between revisions
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[[Image:1i9f.gif|left|200px]] | [[Image:1i9f.gif|left|200px]] | ||
'''STRUCTURAL CHARACTERIZATION OF THE COMPLEX OF THE REV RESPONSE ELEMENT RNA WITH A SELECTED PEPTIDE''' | {{Structure | ||
|PDB= 1i9f |SIZE=350|CAPTION= <scene name='initialview01'>1i9f</scene> | |||
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'''STRUCTURAL CHARACTERIZATION OF THE COMPLEX OF THE REV RESPONSE ELEMENT RNA WITH A SELECTED PEPTIDE''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1I9F is a [ | 1I9F is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I9F OCA]. | ||
==Reference== | ==Reference== | ||
Structural characterization of the complex of the Rev response element RNA with a selected peptide., Zhang Q, Harada K, Cho HS, Frankel AD, Wemmer DE, Chem Biol. 2001 May;8(5):511-20. PMID:[http:// | Structural characterization of the complex of the Rev response element RNA with a selected peptide., Zhang Q, Harada K, Cho HS, Frankel AD, Wemmer DE, Chem Biol. 2001 May;8(5):511-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11358697 11358697] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Cho, H S.]] | [[Category: Cho, H S.]] | ||
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[[Category: solution structure]] | [[Category: solution structure]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:47:18 2008'' | ||
Revision as of 09:47, 20 March 2008
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STRUCTURAL CHARACTERIZATION OF THE COMPLEX OF THE REV RESPONSE ELEMENT RNA WITH A SELECTED PEPTIDE
Overview
INTRODUCTION: The RSG-1.2 peptide was selected for specific binding to the Rev response element RNA, as the natural Rev peptide does. The RSG-1.2 sequence has features incompatible with the helical structure of the bound Rev peptide, indicating that it must bind in a different conformation. RESULTS: The binding of the RSG-1.2 peptide to the Rev response element RNA was characterized using multinuclear, multidimensional NMR. The RSG-1.2 peptide is shown to bind with the N-terminal segment of the peptide along the major groove in an extended conformation and turn preceding a C-terminal helical segment, which crosses the RNA groove in the region widened by the presence of purine-purine base pairs. These features make the details of the bound state rather different than that of the Rev peptide which targets the same RNA sequence binding as a single helix along the groove axis. CONCLUSIONS: These studies further demonstrate the versatility of arginine-rich peptides in recognition of specific RNA elements and the lack of conserved structural features in the bound state.
About this Structure
1I9F is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structural characterization of the complex of the Rev response element RNA with a selected peptide., Zhang Q, Harada K, Cho HS, Frankel AD, Wemmer DE, Chem Biol. 2001 May;8(5):511-20. PMID:11358697
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