1ihj: Difference between revisions
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'''Crystal Structure of the N-terminal PDZ domain of InaD in complex with a NorpA C-terminal peptide''' | {{Structure | ||
|PDB= 1ihj |SIZE=350|CAPTION= <scene name='initialview01'>1ihj</scene>, resolution 1.8Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Phospholipase_C Phospholipase C], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.4.3 3.1.4.3] | |||
|GENE= inaD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 Drosophila melanogaster]) | |||
}} | |||
'''Crystal Structure of the N-terminal PDZ domain of InaD in complex with a NorpA C-terminal peptide''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1IHJ is a [ | 1IHJ is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IHJ OCA]. | ||
==Reference== | ==Reference== | ||
Functional relevance of the disulfide-linked complex of the N-terminal PDZ domain of InaD with NorpA., Kimple ME, Siderovski DP, Sondek J, EMBO J. 2001 Aug 15;20(16):4414-22. PMID:[http:// | Functional relevance of the disulfide-linked complex of the N-terminal PDZ domain of InaD with NorpA., Kimple ME, Siderovski DP, Sondek J, EMBO J. 2001 Aug 15;20(16):4414-22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11500369 11500369] | ||
[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Phospholipase C]] | [[Category: Phospholipase C]] | ||
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[[Category: pdz domain]] | [[Category: pdz domain]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:50:30 2008'' | ||
Revision as of 09:50, 20 March 2008
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| 1ihj, resolution 1.8Å | |||||||||||||
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| Gene: | inaD (Drosophila melanogaster) | ||||||||||||
| Activity: | Phospholipase C, with EC number 3.1.4.3 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal Structure of the N-terminal PDZ domain of InaD in complex with a NorpA C-terminal peptide
Overview
In Drosophila, phototransduction is mediated by G(q)-activation of phospholipase C and is a well studied model system for understanding the kinetics of signal initiation, propagation and termination controlled by G proteins. The proper intracellular targeting and spatial arrangement of most proteins involved in fly phototransduction require the multi-domain scaffolding protein InaD, composed almost entirely of five PDZ domains, which independently bind various proteins including NorpA, the relevant phospho lipase C-beta isozyme. We have determined the crystal structure of the N-terminal PDZ domain of InaD bound to a peptide corresponding to the C-terminus of NorpA to 1.8 A resolution. The structure highlights an intermolecular disulfide bond necessary for high affinity interaction as determined by both in vitro and in vivo studies. Since other proteins also possess similar, cysteine-containing consensus sequences for binding PDZ domains, this disulfide-mediated 'dock-and-lock' interaction of PDZ domains with their ligands may be a relatively ubiquitous mode of coordinating signaling pathways.
About this Structure
1IHJ is a Protein complex structure of sequences from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Functional relevance of the disulfide-linked complex of the N-terminal PDZ domain of InaD with NorpA., Kimple ME, Siderovski DP, Sondek J, EMBO J. 2001 Aug 15;20(16):4414-22. PMID:11500369
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