1ij1: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1ij1.jpg|left|200px]] | [[Image:1ij1.jpg|left|200px]] | ||
'''GCN4-pVLT Coiled-coil Trimer with Threonine at the d(12) Position''' | {{Structure | ||
|PDB= 1ij1 |SIZE=350|CAPTION= <scene name='initialview01'>1ij1</scene>, resolution 1.86Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=ACE:ACETYL GROUP'>ACE</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''GCN4-pVLT Coiled-coil Trimer with Threonine at the d(12) Position''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1IJ1 is a [ | 1IJ1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IJ1 OCA]. | ||
==Reference== | ==Reference== | ||
Buried polar residues in coiled-coil interfaces., Akey DL, Malashkevich VN, Kim PS, Biochemistry. 2001 May 29;40(21):6352-60. PMID:[http:// | Buried polar residues in coiled-coil interfaces., Akey DL, Malashkevich VN, Kim PS, Biochemistry. 2001 May 29;40(21):6352-60. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11371197 11371197] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Akey, D L.]] | [[Category: Akey, D L.]] | ||
| Line 20: | Line 29: | ||
[[Category: gcn4]] | [[Category: gcn4]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 11:50:59 2008'' | ||
Revision as of 09:51, 20 March 2008
| |||||||||||||
| 1ij1, resolution 1.86Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | ZN and ACE | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
GCN4-pVLT Coiled-coil Trimer with Threonine at the d(12) Position
Overview
Coiled coils, estimated to constitute 3-5% of the encoded residues in most genomes, are characterized by a heptad repeat, (abcdefg)(n), where the buried a and d positions form the interface between multiple alpha-helices. Although generally hydrophobic, a substantial fraction ( approximately 20%) of these a- and d-position residues are polar or charged. We constructed variants of the well-characterized coiled coil GCN4-p1 with a single polar residue (Asn, Gln, Ser, or Thr) at either an a or a d position. The stability and oligomeric specificity of each variant were measured, and crystal structures of coiled-coil trimers with threonine or serine at either an a or a d position were determined. The structures show how single polar residues in the interface affect not only local packing, but also overall coiled-coil geometry as seen by changes in the Crick supercoil parameters and core cavity volumes.
About this Structure
1IJ1 is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
Buried polar residues in coiled-coil interfaces., Akey DL, Malashkevich VN, Kim PS, Biochemistry. 2001 May 29;40(21):6352-60. PMID:11371197
Page seeded by OCA on Thu Mar 20 11:50:59 2008
