1par: Difference between revisions

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[[~~Image~~:1par.~~png|left|200px~~]]

==DNA RECOGNITION BY BETA-SHEETS IN THE ARC REPRESSOR-OPERATOR CRYSTAL STRUCTURE==

== Structural highlights ==

<table><tr><td colspan='2'>[[1par]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PAR FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1par FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1par OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1par RCSB], [http://www.ebi.ac.uk/pdbsum/1par PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

Transcription of the ant gene during lytic growth of bacteriophage P22 (ref. 1) is regulated by the cooperative binding of two Arc repressor dimers to a 21-base-pair operator site. Here we report the co-crystal structure of this Arc tetramer-operator complex at 2.6 A resolution. As expected from genetic and structural studies and from the co-crystal structure of the homologous Escherichia coli MetJ repressor, each Arc dimer uses an antiparallel beta-sheet to recognize bases in the major groove. However, the Arc and MetJ complexes differ in several important ways: the beta-sheet-DNA interactions of Arc are far less symmetrical; DNA binding by Arc is accompanied by important conformational changes in the beta-sheet; and Arc uses a different part of its protein surface for dimer-dimer interactions.

~~{{STRUCTURE_1par| PDB=1par | SCENE= }}~~

DNA recognition by beta-sheets in the Arc repressor-operator crystal structure.,Raumann BE, Rould MA, Pabo CO, Sauer RT Nature. 1994 Feb 24;367(6465):754-7. PMID:8107872<ref>PMID:8107872</ref>

~~===DNA RECOGNITION BY BETA-SHEETS IN THE ARC REPRESSOR-OPERATOR CRYSTAL STRUCTURE===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_8107872}}~~

== References ==

~~==About this Structure==~~

__TOC__

~~[[1par]] is~~ a ~~6 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1PAR OCA].~~

</StructureSection>

==~~Reference~~==

<~~ref group="xtra">PMID:008107872<~~/~~ref><ref group="xtra">PMID:011734033</ref~~><~~references group="xtra"~~/>

[[Category: Enterobacteria phage p22]]

[[Category: Pabo, C O.]]

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@@ Line 1: / Line 1: @@
-[[Image:1par.png|left|200px]]
+==DNA RECOGNITION BY BETA-SHEETS IN THE ARC REPRESSOR-OPERATOR CRYSTAL STRUCTURE==
+<StructureSection load='1par' size='340' side='right' caption='[[1par]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1par]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1PAR FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1par FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1par OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1par RCSB], [http://www.ebi.ac.uk/pdbsum/1par PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Transcription of the ant gene during lytic growth of bacteriophage P22 (ref. 1) is regulated by the cooperative binding of two Arc repressor dimers to a 21-base-pair operator site. Here we report the co-crystal structure of this Arc tetramer-operator complex at 2.6 A resolution. As expected from genetic and structural studies and from the co-crystal structure of the homologous Escherichia coli MetJ repressor, each Arc dimer uses an antiparallel beta-sheet to recognize bases in the major groove. However, the Arc and MetJ complexes differ in several important ways: the beta-sheet-DNA interactions of Arc are far less symmetrical; DNA binding by Arc is accompanied by important conformational changes in the beta-sheet; and Arc uses a different part of its protein surface for dimer-dimer interactions.
-{{STRUCTURE_1par|  PDB=1par  |  SCENE=  }}
+DNA recognition by beta-sheets in the Arc repressor-operator crystal structure.,Raumann BE, Rould MA, Pabo CO, Sauer RT Nature. 1994 Feb 24;367(6465):754-7. PMID:8107872<ref>PMID:8107872</ref>
-===DNA RECOGNITION BY BETA-SHEETS IN THE ARC REPRESSOR-OPERATOR CRYSTAL STRUCTURE===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_8107872}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[1par]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacteria_phage_p22 Enterobacteria phage p22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PAR OCA].
+</StructureSection>
-==Reference==
-<ref group="xtra">PMID:008107872</ref><ref group="xtra">PMID:011734033</ref><references group="xtra"/>
 [[Category: Enterobacteria phage p22]]
 [[Category: Pabo, C O.]]