2lm9: Difference between revisions
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[[ | ==The NMR structure of a major allergen from dust mite== | ||
<StructureSection load='2lm9' size='340' side='right' caption='[[2lm9]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2lm9]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Blomia_tropicalis Blomia tropicalis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LM9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LM9 FirstGlance]. <br> | |||
</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lm9 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2lm9 RCSB], [http://www.ebi.ac.uk/pdbsum/2lm9 PDBsum]</span></td></tr> | |||
<table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Blo t 21 is a paralogue of the group 5 allergen, Blo t 5, a major allergen from the dust mite Blomia tropicalis. Blo t 21 has moderate sequence identity (40.7%) to Blo t 5 and low-to-moderate cross-reactivity to Blo t 5. In B. tropicalis, the most prevalent and allergenic allergens are in the order of Blo t 21, Blo t 5 and Blo t 7. Here, we determined the NMR solution structure of Blo t 21 which represents the first structure of the group 21 dust mite allergen. The structure of Blo t 21 closely resembles the structures of Blo t 5 and Der p 5, comprising three anti-parallel alpha-helices arranged in a helical bundle. Using site-directed mutagenesis and specific IgE binding ELISA, Blo t 21 was found to contain both conserved and unique charged IgE eptope residues at the L2 loop region and on helix alpha3. Cross-inhibition assays confirmed that Blo t 21 has a low-to-moderate cross-reactivity with Blo t 5 and Der p 5 and represents a novel group of major allergen in B. tropicalis. In addition to group 5 allergens, Blo t 21 has also a low-to-moderate cross-reactivity with group 21 allergens from Dermatophagoides mites, confirming that B. tropicalis is a major and distinct source of dust mite allergens. | |||
NMR structure and IgE epitopes of Blo t 21, a major dust mite allergen from Blomia tropicalis.,Tan KW, Ong TC, Gao YF, Tiong YS, Wong KN, Chew FT, Mok YK J Biol Chem. 2012 Aug 10. PMID:22887997<ref>PMID:22887997</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
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</StructureSection> | |||
== | |||
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[[Category: Blomia tropicalis]] | [[Category: Blomia tropicalis]] | ||
[[Category: Mok, Y K.]] | [[Category: Mok, Y K.]] | ||
Revision as of 07:02, 9 June 2014
The NMR structure of a major allergen from dust mite
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