4e48: Difference between revisions
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[[ | ==Structure of 20mer double-helical RNA composed of CUG/CUG-repeats== | ||
<StructureSection load='4e48' size='340' side='right' caption='[[4e48]], [[Resolution|resolution]] 2.50Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4e48]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E48 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E48 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4e5c|4e5c]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4e48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4e48 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4e48 RCSB], [http://www.ebi.ac.uk/pdbsum/4e48 PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Human genetic trinucleotide repeat expansion diseases (TREDs) are characterized by triplet repeat expansions, most frequently found as CNG-tracts in genome. At RNA level, such expansions suggestively result in formation of double-helical hairpins that become a potential source for small RNAs involved in RNA interference (RNAi). Here, we present three crystal structures of RNA fragments composed of triplet repeats CUG and CGG/CUG, as well as two crystal structures of same triplets in a protein-bound state. We show that both 20mer pG(CUG)(6)C and 19mer pGG(CGG)(3)(CUG)(2)CC form A-RNA duplexes, in which U.U or G.U mismatches are flanked/stabilized by two consecutive Watson-Crick G.C base pairs resulting in high-stacking GpC steps in every third position of the duplex. Despite interruption of this regularity in another 19mer, p(CGG)(3)C(CUG)(3), the oligonucleotide still forms regular double-helical structure, characterized, however, by 12 bp (rather than 11 bp) per turn. Analysis of newly determined molecular structures reveals the dynamic aspects of U.U and G.U mismatching within CNG-repetitive A-RNA and in a protein-bound state, as well as identifies an additional mode of U.U pairing essential for its dynamics and sheds the light on possible role of regularity of trinucleotide repeats for double-helical RNA structure. Findings are important for understanding the structural behavior of CNG-repetitive RNA double helices implicated in TREDs. | |||
Structural dynamics of double-helical RNAs composed of CUG/CUG- and CUG/CGG-repeats.,Tamjar J, Katorcha E, Popov A, Malinina L J Biomol Struct Dyn. 2012 Jun 25. PMID:22731704<ref>PMID:22731704</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Katorcha, E]] | |||
== | [[Category: Malinina, L]] | ||
< | |||
[[Category: Katorcha, E | |||
[[Category: Malinina, L | |||
[[Category: Rna]] | [[Category: Rna]] | ||
[[Category: Sirna]] | [[Category: Sirna]] | ||
[[Category: Trinucleotide repeat expansion]] | [[Category: Trinucleotide repeat expansion]] | ||
Revision as of 14:33, 4 January 2015
Structure of 20mer double-helical RNA composed of CUG/CUG-repeats
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