1jou: Difference between revisions
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[[Image:1jou.jpg|left|200px]] | [[Image:1jou.jpg|left|200px]] | ||
'''Crystal Structure of Native S195A Thrombin with an Unoccupied Active Site''' | {{Structure | ||
|PDB= 1jou |SIZE=350|CAPTION= <scene name='initialview01'>1jou</scene>, resolution 1.80Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NDG:2-(ACETYLAMINO)-2-DEOXY-A-D-GLUCOPYRANOSE'>NDG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> and <scene name='pdbligand=ACY:ACETIC ACID'>ACY</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal Structure of Native S195A Thrombin with an Unoccupied Active Site''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1JOU is a [ | 1JOU is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JOU OCA]. | ||
==Reference== | ==Reference== | ||
The molecular basis of thrombin allostery revealed by a 1.8 A structure of the "slow" form., Huntington JA, Esmon CT, Structure. 2003 Apr;11(4):469-79. PMID:[http:// | The molecular basis of thrombin allostery revealed by a 1.8 A structure of the "slow" form., Huntington JA, Esmon CT, Structure. 2003 Apr;11(4):469-79. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12679024 12679024] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
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[[Category: thrombin]] | [[Category: thrombin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:06:20 2008'' | ||
Revision as of 10:06, 20 March 2008
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| 1jou, resolution 1.80Å | |||||||||||||
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| Ligands: | NDG, NA, GOL and ACY | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal Structure of Native S195A Thrombin with an Unoccupied Active Site
Overview
Thrombin participates in its own positive and negative feedback loops, and its allosteric state helps determine the hemostatic balance. Here we present the 1.8 A crystallographic structure of S195A thrombin in two conformational states: active site occupied and active site free. The active site-occupied form shows how thrombin can accommodate substrates, such as protein C. The active site-free form is in a previously unobserved closed conformation of thrombin, which satisfies all the conditions of the so-called "slow" form. A mechanism of allostery is revealed, which relies on the concerted movement of the disulphide bond between Cys168 and 182 and aromatic residues Phe227, Trp215, and Trp60d. These residues constitute an allosteric switch, which is flipped directly through sodium binding, resulting in the fast form with an open active site.
Disease
Known diseases associated with this structure: Dysprothrombinemia OMIM:[176930], Hyperprothrombinemia OMIM:[176930], Hypoprothrombinemia OMIM:[176930]
About this Structure
1JOU is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The molecular basis of thrombin allostery revealed by a 1.8 A structure of the "slow" form., Huntington JA, Esmon CT, Structure. 2003 Apr;11(4):469-79. PMID:12679024
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