1jza: Difference between revisions
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[[Image:1jza.gif|left|200px]] | [[Image:1jza.gif|left|200px]] | ||
'''Crystal Structure of Variant 2 Scorpion Toxin from Centruroides sculpturatus Ewing''' | {{Structure | ||
|PDB= 1jza |SIZE=350|CAPTION= <scene name='initialview01'>1jza</scene>, resolution 2.20Å | |||
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'''Crystal Structure of Variant 2 Scorpion Toxin from Centruroides sculpturatus Ewing''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1JZA is a [ | 1JZA is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Centruroides_sculpturatus Centruroides sculpturatus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JZA OCA]. | ||
==Reference== | ==Reference== | ||
Structure of variant 2 scorpion toxin from Centruroides sculpturatus Ewing., Cook WJ, Zell A, Watt DD, Ealick SE, Protein Sci. 2002 Mar;11(3):479-86. PMID:[http:// | Structure of variant 2 scorpion toxin from Centruroides sculpturatus Ewing., Cook WJ, Zell A, Watt DD, Ealick SE, Protein Sci. 2002 Mar;11(3):479-86. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11847271 11847271] | ||
[[Category: Centruroides sculpturatus]] | [[Category: Centruroides sculpturatus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: scorpion toxin]] | [[Category: scorpion toxin]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:10:28 2008'' | ||
Revision as of 10:10, 20 March 2008
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Crystal Structure of Variant 2 Scorpion Toxin from Centruroides sculpturatus Ewing
Overview
Centruroides sculpturatus Ewing variant 2 toxin (CsE-v2) is a neurotoxin isolated from the venom of a scorpion native to the Arizona desert. The structure of CsE-v2 was solved in two different crystal forms using a combination of molecular replacement and multiple isomorphous replacement techniques. Crystals of CsE-v2 display a temperature-dependent, reversible-phase transition near room temperature. At lower temperature the space group changes from P3(2)21 to P3(1)21 with an approximate doubling of the C-axis. The small-cell structure, which has one molecule per asymmetric unit, has an R factor of 0.229 at 2.8 A resolution. The large-cell structure has two molecules per asymmetric unit and was refined at 2.2 A resolution to an R factor of 0.255. CsE-v2 is a rigid, compact structure with four intrachain disulfide bonds. The structure is similar to other long-chain beta neurotoxins, and the largest differences occur in the last six residues. The high-resolution structure of CsE-v2 corrects an error in the reported C-terminal sequence; the terminal tripeptide sequence is Ser 64-Cys 65-Ser 66 rather than Ser 64-Ser 65-Cys 66. Comparison of CsE-v2 with long-chain alpha toxins reveals four insertions and one deletion, as well as additional residues at the N and C termini. Structural alignment of alpha and beta toxins suggests that the primary distinguishing feature between the two classes is the length of the loop between the second and third strands in a three-strand beta sheet. The shorter loop in alpha toxins exposes a critical lysine side chain, whereas the longer loop in beta toxins buries the corresponding basic residue (either arginine or lysine).
About this Structure
1JZA is a Single protein structure of sequence from Centruroides sculpturatus. Full crystallographic information is available from OCA.
Reference
Structure of variant 2 scorpion toxin from Centruroides sculpturatus Ewing., Cook WJ, Zell A, Watt DD, Ealick SE, Protein Sci. 2002 Mar;11(3):479-86. PMID:11847271
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