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[[Image:3vk2.jpg|left|200px]]
==Crystal Structure of L-Methionine gamma-Lyase from Pseudomonas putida C116H Mutant.==
<StructureSection load='3vk2' size='340' side='right' caption='[[3vk2]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3vk2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VK2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3VK2 FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
<tr><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LLP:2-LYSINE(3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHANE)'>LLP</scene></td></tr>
<tr><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2o7c|2o7c]], [[3vk3|3vk3]], [[3vk4|3vk4]]</td></tr>
<tr><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mdeA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=303 Pseudomonas putida])</td></tr>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Methionine_gamma-lyase Methionine gamma-lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.4.1.11 4.4.1.11] </span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3vk2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3vk2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3vk2 RCSB], [http://www.ebi.ac.uk/pdbsum/3vk2 PDBsum]</span></td></tr>
<table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cys116, Lys240&lt;sup&gt;*&lt;/sup&gt;, and Asp241&lt;sup&gt;*&lt;/sup&gt; (asterisks indicate residues from the second subunit of the active dimer) at the active site of L-methionine gamma-lyase of Pseudomonas putida (MGL_Pp) are highly conserved among heterologous MGLs. In a previous study, we found that substitution of Cys116 for His led to a drastic increase in activity toward L-cysteine and a decrease in that toward L-methionine. In this study, we examined some properties of the C116H mutant by kinetic analysis and 3D structural analysis. We assumed that substitution of Cys116 for His broke the original hydrogen-bond network and that this induced a significant effect of Tyr114 as a general acid catalyst, possibly due to the narrow space in the active site. The C116H mutant acquired a novel beta-elimination activity and lead a drastic conformation change in the histidine residue at position 116 by binding the substrate, suggesting that this His residue affects the reaction specificity of C116H. Furthermore, we suggest that Lys240&lt;sup&gt;*&lt;/sup&gt; is important for substrate recognition and structural stability and that Asp241&lt;sup&gt;*&lt;/sup&gt; is also involved in substrate specificity in the elimination reaction. Based on this, we suggest that the hydrogen-bond network among Cys116, Lys240&lt;sup&gt;*&lt;/sup&gt;, and Asp241&lt;sup&gt;*&lt;/sup&gt; contributes to substrate specificity that is, to L-methionine recognition at the active site in MGL_Pp.


{{STRUCTURE_3vk2|  PDB=3vk2  |  SCENE=  }}
The Role of Amino Acid Residues in the Active Site of &lt;small&gt;L&lt;/small&gt;-Methionine gamma-lyase from &lt;i&gt;Pseudomonas putida&lt;/i&gt;.,Fukumoto M, Kudou D, Murano S, Shiba T, Sato D, Tamura T, Harada S, Inagaki K Biosci Biotechnol Biochem. 2012 Jul 23;76(7):1275-84. Epub 2012 Jul 7. PMID:22785484<ref>PMID:22785484</ref>


===Crystal Structure of L-Methionine gamma-Lyase from Pseudomonas putida C116H Mutant.===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_22785484}}
== References ==
 
<references/>
==About this Structure==
__TOC__
[[3vk2]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_putida Pseudomonas putida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3VK2 OCA].
</StructureSection>
[[Category: Methionine gamma-lyase]]
[[Category: Methionine gamma-lyase]]
[[Category: Pseudomonas putida]]
[[Category: Pseudomonas putida]]

Revision as of 06:51, 25 June 2014

Crystal Structure of L-Methionine gamma-Lyase from Pseudomonas putida C116H Mutant.

3vk2, resolution 2.30Å

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