Introduction to Evolutionary Conservation: Difference between revisions
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===Sophisticated Analysis of Conservation: ConSurf=== | ===Sophisticated Analysis of Conservation: ConSurf=== | ||
The above analysis assigns each amino acid in enolase to one of three categories: conserved, similar, or different. This is very simplistic. In contrast, the analysis used in Proteopedia (and in the above image) is sophisticated, using many more sequences, and weighting each sequence in the multiple sequence alignment according to the phylogenetic tree calculated from the alignment. This sophisticated determination of conservation and variability is done by the [[ConSurfDB vs. ConSurf|ConSurf Servers]] (see also a [[ConSurfDB_vs._ConSurf#The_ConSurf-DB_Mechanism|summary of their mechanism]]). ConSurf divides conservation into 9 levels, and colors them as follows: | The above analysis assigns each amino acid in enolase to one of three categories: conserved, similar, or different. This is very simplistic. In contrast, the analysis used in Proteopedia (and in the above image) is sophisticated, using many more sequences, and weighting each sequence in the multiple sequence alignment according to the phylogenetic tree calculated from the alignment. This sophisticated determination of conservation and variability is done by the [[ConSurfDB vs. ConSurf|ConSurf Servers]] (see also a [[ConSurfDB_vs._ConSurf#The_ConSurf-DB_Mechanism|summary of their mechanism]]). ConSurf divides conservation into 9 levels, and colors them as follows: | ||
{{Template:ColorKey_ConSurf_NoGray}} | <center>{{Template:ColorKey_ConSurf_NoGray}}</center> | ||
When ConSurf's colors are applied to the 436 amino acids in the sequence of enolase (based on a multiple sequence alignment containing 150 sequences), this is the result: | When ConSurf's colors are applied to the 436 amino acids in the sequence of enolase (based on a multiple sequence alignment containing 150 sequences), this is the result: | ||
