3o5w: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older editNewer edit →
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
Line 1: Line 1:
[[Image:3o5w.png|left|200px]]
==Binding of kinetin in the active site of mistletoe lectin I==
<StructureSection load='3o5w' size='340' side='right' caption='[[3o5w]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3o5w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O5W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3O5W FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=H35:N-(FURAN-2-YLMETHYL)-7H-PURIN-6-AMINE'>H35</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1m2t|1m2t]], [[2r9k|2r9k]], [[3d7w|3d7w]]</td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/rRNA_N-glycosylase rRNA N-glycosylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.2.22 3.2.2.22] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3o5w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3o5w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3o5w RCSB], [http://www.ebi.ac.uk/pdbsum/3o5w PDBsum]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of the ribosome inhibiting protein Mistletoe Lectin I (ML-I) derived from the European mistletoe, Viscum album, in complex with kinetin has been refined at 2.7A resolution. Suitably large crystals of ML-I were obtained applying the counter diffusion method using the Gel Tube R Crystallization Kit (GT-R) on board the Russian Service Module on the international space station ISS within the GCF mission No. 6, arranged by the Japanese aerospace exploration agency (JAXA). Hexagonal bi-pyramidal crystals were grown during three months under microgravity. Before data collection the crystals were soaked in a saturated solution of kinetin and diffraction data to 2.7A were collected using synchrotron radiation and cryogenic techniques. The atomic model was refined and revealed a single kinetin molecule in the ribosome inactivation site of ML-I. The complex demonstrates the feasibility of mistletoe to bind plant hormones out of the host regulation system as part of a self protection mechanism.


{{STRUCTURE_3o5w|  PDB=3o5w  |  SCENE=  }}
Binding of the plant hormone kinetin in the active site of Mistletoe Lectin I from Viscum album.,Malecki PH, Rypniewski W, Szymanski M, Barciszewski J, Meyer A Biochim Biophys Acta. 2012 Feb;1824(2):334-8. doi: 10.1016/j.bbapap.2011.10.013. , Epub 2011 Oct 28. PMID:22064121<ref>PMID:22064121</ref>


===Binding of kinetin in the active site of mistletoe lectin I===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_22064121}}
== References ==
 
<references/>
==About this Structure==
__TOC__
[[3o5w]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Viscum_album Viscum album]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3O5W OCA].
</StructureSection>
[[Category: Viscum album]]
[[Category: Viscum album]]
[[Category: RRNA N-glycosylase]]
[[Category: RRNA N-glycosylase]]
[[Category: Barciszewski, J.]]
[[Category: Barciszewski, J]]
[[Category: Betzel, C.]]
[[Category: Betzel, C]]
[[Category: Malecki, P H.]]
[[Category: Malecki, P H]]
[[Category: Meyer, A.]]
[[Category: Meyer, A]]
[[Category: Rypniewski, W.]]
[[Category: Rypniewski, W]]
[[Category: Szymanski, M.]]
[[Category: Szymanski, M]]
[[Category: Active site]]
[[Category: Active site]]
[[Category: Cytokinin]]
[[Category: Cytokinin]]
Line 23: Line 34:
[[Category: Microgravity]]
[[Category: Microgravity]]
[[Category: Ribosome inactivating protein]]
[[Category: Ribosome inactivating protein]]
[[Category: Viscum album]]
Retrieved from "https://proteopedia.org/3o5w"