1kix: Difference between revisions
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[[Image:1kix.gif|left|200px]] | [[Image:1kix.gif|left|200px]] | ||
'''Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA''' | {{Structure | ||
|PDB= 1kix |SIZE=350|CAPTION= <scene name='initialview01'>1kix</scene>, resolution 2.70Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |||
|ACTIVITY= | |||
|GENE= MAC-56A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=200597 Sterkiella nova]) | |||
}} | |||
'''Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1KIX is a [ | 1KIX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sterkiella_nova Sterkiella nova]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KIX OCA]. | ||
==Reference== | ==Reference== | ||
Dimeric structure of the Oxytricha nova telomere end-binding protein alpha-subunit bound to ssDNA., Peersen OB, Ruggles JA, Schultz SC, Nat Struct Biol. 2002 Mar;9(3):182-7. PMID:[http:// | Dimeric structure of the Oxytricha nova telomere end-binding protein alpha-subunit bound to ssDNA., Peersen OB, Ruggles JA, Schultz SC, Nat Struct Biol. 2002 Mar;9(3):182-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11836536 11836536] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sterkiella nova]] | [[Category: Sterkiella nova]] | ||
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[[Category: Schultz, S C.]] | [[Category: Schultz, S C.]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: dna-protein | [[Category: dna-protein interaction]] | ||
[[Category: single stranded dna]] | [[Category: single stranded dna]] | ||
[[Category: ssdna]] | [[Category: ssdna]] | ||
[[Category: telomere binding protein]] | [[Category: telomere binding protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:18:06 2008'' | ||
Revision as of 10:18, 20 March 2008
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| 1kix, resolution 2.70Å | |||||||||||||
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| Ligands: | SO4 | ||||||||||||
| Gene: | MAC-56A (Sterkiella nova) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Dimeric Structure of the O. nova Telomere End Binding Protein Alpha Subunit with Bound ssDNA
Overview
Telomeres are the specialized protein--DNA complexes that cap and protect the ends of linear eukaryotic chromosomes. The extreme 3' end of the telomeric DNA in Oxytricha nova is bound by a two-subunit sequence-specific and 3' end-specific protein called the telomere end-binding protein (OnTEBP). Here we describe the crystal structure of the alpha-subunit of OnTEBP in complex with T4G4 single-stranded telomeric DNA. This structure shows an (alpha--ssDNA)2 homodimer with a large approximately 7,000 A2 protein--protein interface in which the domains of alpha are rearranged extensively from their positions in the structure of an alpha--beta--ssDNA ternary complex. The (alpha--ssDNA)2 complex can bind two telomeres on opposite sides of the dimer and, thus, acts as a protein mediator of telomere--telomere associations. The structures of the (alpha--ssDNA)2 dimer presented here and the previously described alpha--beta--ssDNA complex demonstrate that OnTEBP forms multiple telomeric complexes that potentially mediate the assembly and disassembly of higher order telomeric structures.
About this Structure
1KIX is a Single protein structure of sequence from Sterkiella nova. Full crystallographic information is available from OCA.
Reference
Dimeric structure of the Oxytricha nova telomere end-binding protein alpha-subunit bound to ssDNA., Peersen OB, Ruggles JA, Schultz SC, Nat Struct Biol. 2002 Mar;9(3):182-7. PMID:11836536
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