1kou: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
[[Image:1kou.gif|left|200px]] | [[Image:1kou.gif|left|200px]] | ||
'''Crystal Structure of the Photoactive Yellow Protein Reconstituted with Caffeic Acid at 1.16 A Resolution''' | {{Structure | ||
|PDB= 1kou |SIZE=350|CAPTION= <scene name='initialview01'>1kou</scene>, resolution 1.16Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=DHC:CAFFEIC+ACID'>DHC</scene> and <scene name='pdbligand=NBU:N-BUTANE'>NBU</scene> | |||
|ACTIVITY= | |||
|GENE= PYP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1053 Halorhodospira halophila]) | |||
}} | |||
'''Crystal Structure of the Photoactive Yellow Protein Reconstituted with Caffeic Acid at 1.16 A Resolution''' | |||
==Overview== | ==Overview== | ||
| Line 7: | Line 16: | ||
==About this Structure== | ==About this Structure== | ||
1KOU is a [ | 1KOU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KOU OCA]. | ||
==Reference== | ==Reference== | ||
Structure of the photoactive yellow protein reconstituted with caffeic acid at 1.16 A resolution., van Aalten DM, Crielaard W, Hellingwerf KJ, Joshua-Tor L, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):585-90. Epub 2002, Mar 22. PMID:[http:// | Structure of the photoactive yellow protein reconstituted with caffeic acid at 1.16 A resolution., van Aalten DM, Crielaard W, Hellingwerf KJ, Joshua-Tor L, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):585-90. Epub 2002, Mar 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11914481 11914481] | ||
[[Category: Halorhodospira halophila]] | [[Category: Halorhodospira halophila]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| Line 21: | Line 30: | ||
[[Category: photoreceptor]] | [[Category: photoreceptor]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:20:13 2008'' | ||
Revision as of 10:20, 20 March 2008
| |||||||||||||
| 1kou, resolution 1.16Å | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Ligands: | DHC and NBU | ||||||||||||
| Gene: | PYP (Halorhodospira halophila) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal Structure of the Photoactive Yellow Protein Reconstituted with Caffeic Acid at 1.16 A Resolution
Overview
A structural study is described of the photoactive yellow protein (PYP) reconstituted with the chromophore derivative 3,4-dihydroxycinnamic acid. The crystal structure of PYP reconstituted with this chromophore at 1.16 A resolution is reported in space group P6(5). This is the first high-resolution structure of a photoreceptor containing a modified chromophore. The introduction of an extra hydroxyl group in the native chromophore (i.e. p-coumaric acid) appears to perturb the structure of the hybrid yellow protein only slightly. The chromophore is bound by the protein in two different conformations, separated by a rotation of 180 degrees of the catechol ring. In combination with available spectroscopic data, it is concluded that the caffeic acid chromophore binds to the protein in a strained conformation, which leads to a faster ejection from the chromophore-binding pocket upon pB formation.
About this Structure
1KOU is a Single protein structure of sequence from Halorhodospira halophila. Full crystallographic information is available from OCA.
Reference
Structure of the photoactive yellow protein reconstituted with caffeic acid at 1.16 A resolution., van Aalten DM, Crielaard W, Hellingwerf KJ, Joshua-Tor L, Acta Crystallogr D Biol Crystallogr. 2002 Apr;58(Pt 4):585-90. Epub 2002, Mar 22. PMID:11914481
Page seeded by OCA on Thu Mar 20 12:20:13 2008
