1kwh: Difference between revisions
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'''Structure Analysis AlgQ2, a Macromolecule(alginate)-Binding Periplasmic Protein of Sphingomonas sp. A1.''' | {{Structure | ||
|PDB= 1kwh |SIZE=350|CAPTION= <scene name='initialview01'>1kwh</scene>, resolution 2.00Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=CA:CALCIUM ION'>CA</scene> | |||
|ACTIVITY= | |||
|GENE= algQ2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=28214 Sphingomonas sp.]) | |||
}} | |||
'''Structure Analysis AlgQ2, a Macromolecule(alginate)-Binding Periplasmic Protein of Sphingomonas sp. A1.''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1KWH is a [ | 1KWH is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Sphingomonas_sp. Sphingomonas sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1KWH OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp. A1 at 2.0A resolution., Momma K, Mikami B, Mishima Y, Hashimoto W, Murata K, J Mol Biol. 2002 Mar 8;316(5):1051-9. PMID:[http:// | Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp. A1 at 2.0A resolution., Momma K, Mikami B, Mishima Y, Hashimoto W, Murata K, J Mol Biol. 2002 Mar 8;316(5):1051-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11884143 11884143] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sphingomonas sp.]] | [[Category: Sphingomonas sp.]] | ||
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[[Category: binding protein]] | [[Category: binding protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:23:07 2008'' | ||
Revision as of 10:23, 20 March 2008
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| 1kwh, resolution 2.00Å | |||||||||||||
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| Ligands: | CA | ||||||||||||
| Gene: | algQ2 (Sphingomonas sp.) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Structure Analysis AlgQ2, a Macromolecule(alginate)-Binding Periplasmic Protein of Sphingomonas sp. A1.
Overview
Sphingomonas sp. A1 possesses a high molecular mass (average 25,700 Da) alginate uptake system mediated by a novel pit-dependent ABC transporter. The X-ray crystallographic structure of AlgQ2 (57,200 Da), an alginate-binding protein in the system, was determined by the multiple isomorphous replacement method and refined at 2.0 A resolution with a final R-factor of 18.3% for 15 to 2.0 A resolution data. The refined structure of AlgQ2 was comprised of 492 amino acid residues, 172 water molecules, and one calcium ion. AlgQ2 was composed of two globular domains with a deep cleft between them, which is expected to be the alginate-binding site. The overall structure is basically similar to that of maltose/maltodextrin-binding protein, except for the presence of an N2-subdomain. The entire calcium ion-binding site is similar to the site in the EF-hand motif, but comprises a ten residue loop. This calcium ion-binding site is about 40 A away from the alginate-binding site.
About this Structure
1KWH is a Single protein structure of sequence from Sphingomonas sp.. Full crystallographic information is available from OCA.
Reference
Crystal structure of AlgQ2, a macromolecule (alginate)-binding protein of Sphingomonas sp. A1 at 2.0A resolution., Momma K, Mikami B, Mishima Y, Hashimoto W, Murata K, J Mol Biol. 2002 Mar 8;316(5):1051-9. PMID:11884143
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