3rcz: Difference between revisions
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[[ | ==Rad60 SLD2 Ubc9 Complex== | ||
<StructureSection load='3rcz' size='340' side='right' caption='[[3rcz]], [[Resolution|resolution]] 1.90Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3rcz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RCZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RCZ FirstGlance]. <br> | |||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3goe|3goe]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rad60, SPBC1921.02 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]), hus5, ubc9, SPAC30D11.13 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rcz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rcz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3rcz RCSB], [http://www.ebi.ac.uk/pdbsum/3rcz PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Global sumoylation, SUMO chain formation and genome stabilization are all outputs generated by a limited repertoire of enzymes. Mechanisms driving selectivity for each of these processes are largely uncharacterized. Here, through crystallographic analyses we show that the SUMO E2 Ubc9 forms a non-covalent complex with a SUMO-like domain of Rad60 (SLD2). Ubc9:SLD2 and Ubc9:SUMO non-covalent complexes are structurally analogous, suggesting that differential recruitment of Ubc9 by SUMO or Rad60 provides a novel means for such selectivity. Indeed, deconvoluting Ubc9 function by disrupting either the Ubc9:SLD2 or Ubc9:SUMO non-covalent complex reveals distinct roles in facilitating sumoylation. Ubc9:SLD2 acts in the Nse2 SUMO E3 ligase-dependent pathway for DNA repair, whereas Ubc9:SUMO instead promotes global sumoylation and chain formation, via the Pli1 E3 SUMO ligase. Moreover, this Pli1-dependent SUMO chain formation causes the genome instability phenotypes of SUMO-targeted ubiquitin ligase (STUbL) mutants. Overall, we determine that unexpectedly, Ubc9 non-covalent partner choice dictates the role of sumoylation in distinct cellular pathways. | |||
DNA Repair and Global Sumoylation are Regulated by Distinct Ubc9 Non-covalent Complexes.,Prudden J, Perry JJ, Nie M, Vashisht AA, Arvai AS, Hitomi C, Guenther G, Wohlschlegel JA, Tainer JA, Boddy MN Mol Cell Biol. 2011 Mar 28. PMID:21444718<ref>PMID:21444718</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | ==See Also== | ||
*[[SUMO conjugating enzyme Ubc9|SUMO conjugating enzyme Ubc9]] | *[[SUMO conjugating enzyme Ubc9|SUMO conjugating enzyme Ubc9]] | ||
== References == | |||
== | <references/> | ||
< | __TOC__ | ||
</StructureSection> | |||
[[Category: Schizosaccharomyces pombe]] | [[Category: Schizosaccharomyces pombe]] | ||
[[Category: Arvai, A S | [[Category: Arvai, A S]] | ||
[[Category: Perry, J J.P | [[Category: Perry, J J.P]] | ||
[[Category: Tainer, J A | [[Category: Tainer, J A]] | ||
[[Category: Protein binding-ligase complex]] | [[Category: Protein binding-ligase complex]] | ||
[[Category: Protein:protein interaction]] | [[Category: Protein:protein interaction]] | ||
[[Category: Sumo-like domain]] | [[Category: Sumo-like domain]] | ||
[[Category: Ubc9]] | [[Category: Ubc9]] | ||
