1lwr: Difference between revisions
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[[Image:1lwr.gif|left|200px]] | [[Image:1lwr.gif|left|200px]] | ||
'''Solution structure of the NCAM fibronectin type III module 2''' | {{Structure | ||
|PDB= 1lwr |SIZE=350|CAPTION= <scene name='initialview01'>1lwr</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= Ncam1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | |||
}} | |||
'''Solution structure of the NCAM fibronectin type III module 2''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1LWR is a [ | 1LWR is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1LWR OCA]. | ||
==Reference== | ==Reference== | ||
Structural basis for a direct interaction between FGFR1 and NCAM and evidence for a regulatory role of ATP., Kiselyov VV, Skladchikova G, Hinsby AM, Jensen PH, Kulahin N, Soroka V, Pedersen N, Tsetlin V, Poulsen FM, Berezin V, Bock E, Structure. 2003 Jun;11(6):691-701. PMID:[http:// | Structural basis for a direct interaction between FGFR1 and NCAM and evidence for a regulatory role of ATP., Kiselyov VV, Skladchikova G, Hinsby AM, Jensen PH, Kulahin N, Soroka V, Pedersen N, Tsetlin V, Poulsen FM, Berezin V, Bock E, Structure. 2003 Jun;11(6):691-701. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12791257 12791257] | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: fibronectin type iii module]] | [[Category: fibronectin type iii module]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:36:08 2008'' | ||
Revision as of 10:36, 20 March 2008
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| Gene: | Ncam1 (Rattus norvegicus) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Solution structure of the NCAM fibronectin type III module 2
Overview
The neural cell adhesion molecule (NCAM) promotes axonal outgrowth, presumably through an interaction with the fibroblast growth factor receptor (FGFR). NCAM also has a little-understood ATPase activity. We here demonstrate for the first time a direct interaction between NCAM (fibronectin type III [F3] modules 1 and 2) and FGFR1 (Ig modules 2 and 3) by surface plasmon resonance (SPR) analysis. The structure of the NCAM F3 module 2 was determined by NMR and the module was shown by NMR to interact with the FGFR1 Ig module 3 and ATP. The NCAM sites binding to FGFR and ATP were found to overlap and ATP was shown by SPR to inhibit the NCAM-FGFR binding, indicating that ATP probably regulates the NCAM-FGFR interaction. Furthermore, we demonstrate that the NCAM module was able to induce activation (phosphorylation) of FGFR and to stimulate neurite outgrowth. In contrast, ATP inhibited neurite outgrowth induced by the module.
About this Structure
1LWR is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structural basis for a direct interaction between FGFR1 and NCAM and evidence for a regulatory role of ATP., Kiselyov VV, Skladchikova G, Hinsby AM, Jensen PH, Kulahin N, Soroka V, Pedersen N, Tsetlin V, Poulsen FM, Berezin V, Bock E, Structure. 2003 Jun;11(6):691-701. PMID:12791257
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