1n69: Difference between revisions
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[[Image:1n69.gif|left|200px]] | [[Image:1n69.gif|left|200px]] | ||
'''Crystal structure of human saposin B''' | {{Structure | ||
|PDB= 1n69 |SIZE=350|CAPTION= <scene name='initialview01'>1n69</scene>, resolution 2.20Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=PEH:DI-STEAROYL-3-SN-PHOSPHATIDYLETHANOLAMINE'>PEH</scene> | |||
|ACTIVITY= | |||
|GENE= Prosaposin, PSAP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |||
}} | |||
'''Crystal structure of human saposin B''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1N69 is a [ | 1N69 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N69 OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of saposin B reveals a dimeric shell for lipid binding., Ahn VE, Faull KF, Whitelegge JP, Fluharty AL, Prive GG, Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):38-43. Epub 2002 Dec 23. PMID:[http:// | Crystal structure of saposin B reveals a dimeric shell for lipid binding., Ahn VE, Faull KF, Whitelegge JP, Fluharty AL, Prive GG, Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):38-43. Epub 2002 Dec 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12518053 12518053] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: lipid binding protein]] | [[Category: lipid binding protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:52:48 2008'' | ||
Revision as of 10:52, 20 March 2008
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| 1n69, resolution 2.20Å | |||||||||||||
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| Ligands: | PEH | ||||||||||||
| Gene: | Prosaposin, PSAP (Homo sapiens) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of human saposin B
Overview
Saposin B is a small, nonenzymatic glycosphingolipid activator protein required for the breakdown of cerebroside sulfates (sulfatides) within the lysosome. The protein can extract target lipids from membranes, forming soluble protein-lipid complexes that are recognized by arylsulfatase A. The crystal structure of human saposin B reveals an unusual shell-like dimer consisting of a monolayer of alpha-helices enclosing a large hydrophobic cavity. Although the secondary structure of saposin B is similar to that of the known monomeric members of the saposin-like superfamily, the helices are repacked into a different tertiary arrangement to form the homodimer. A comparison of the two forms of the saposin B dimer suggests that extraction of target lipids from membranes involves a conformational change that facilitates access to the inner cavity.
Disease
Known diseases associated with this structure: Combined SAP deficiency OMIM:[176801], Gaucher disease, atypical OMIM:[176801], Krabbe disease, atypical OMIM:[176801], Metachromatic leukodystrophy due to SAP-b deficiency OMIM:[176801]
About this Structure
1N69 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of saposin B reveals a dimeric shell for lipid binding., Ahn VE, Faull KF, Whitelegge JP, Fluharty AL, Prive GG, Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):38-43. Epub 2002 Dec 23. PMID:12518053
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