3eh8: Difference between revisions

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~~[[Image:3eh8.png|left|200px]]~~

===Crystal structure of Y2 I-AniI variant (F13Y/S111Y)/DNA complex with calcium===

~~{{ABSTRACT_PUBMED_19103658}}~~

==Function==

[[http://www.uniprot.org/uniprot/ANI1_EMEND ANI1_EMEND]] Mitochondrial DNA endonuclease and mRNA maturase involved in intron homing and required for splicing of the cytochrome b (cobA) gene intron, containing its own coding sequence. The protein stimulates the intrinsic ribozyme activity of the intron through binding to and stabilizing specific secondary and tertiary structure elements in the RNA. As an endonuclease it introduces a specific double-strand break at the junction of the two exons the cobA gene and thus mediates the insertion of an intron, containing its own coding sequence (group I intron), into an intronless gene. Recognizes with limited specificity and cleaves the sequence 5'-GAGGAGGTTTCTCTGTA-3'. The proteins RNA and DNA recognition and binding surfaces are independent.<ref>PMID:9256423</ref> <ref>PMID:10512698</ref> <ref>PMID:12758073</ref>

==About this Structure==

[[3eh8]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EH8 OCA].

~~==See Also==~~

*[[Endonuclease|Endonuclease]]

==Reference==

[[Category: Emericella nidulans]]

[[Category: Stoddard, B L.]]

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-[[Image:3eh8.png|left|200px]]
 {{STRUCTURE_3eh8|  PDB=3eh8  |  SCENE=  }}
 ===Crystal structure of Y2 I-AniI variant (F13Y/S111Y)/DNA complex with calcium===
+{{ABSTRACT_PUBMED_19103658}}
-{{ABSTRACT_PUBMED_19103658}}
+==Function==
+[[http://www.uniprot.org/uniprot/ANI1_EMEND ANI1_EMEND]] Mitochondrial DNA endonuclease and mRNA maturase involved in intron homing and required for splicing of the cytochrome b (cobA) gene intron, containing its own coding sequence. The protein stimulates the intrinsic ribozyme activity of the intron through binding to and stabilizing specific secondary and tertiary structure elements in the RNA. As an endonuclease it introduces a specific double-strand break at the junction of the two exons the cobA gene and thus mediates the insertion of an intron, containing its own coding sequence (group I intron), into an intronless gene. Recognizes with limited specificity and cleaves the sequence 5'-GAGGAGGTTTCTCTGTA-3'. The proteins RNA and DNA recognition and binding surfaces are independent.<ref>PMID:9256423</ref> <ref>PMID:10512698</ref> <ref>PMID:12758073</ref>
 ==About this Structure==
 [[3eh8]] is a 9 chain structure with sequence from [http://en.wikipedia.org/wiki/Emericella_nidulans Emericella nidulans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EH8 OCA].
-==See Also==
-*[[Endonuclease|Endonuclease]]
 ==Reference==
-<ref group="xtra">PMID:019103658</ref><references group="xtra"/>
+<ref group="xtra">PMID:019103658</ref><references group="xtra"/><references/>
 [[Category: Emericella nidulans]]
 [[Category: Stoddard, B L.]]