1ncz: Difference between revisions
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[[Image:1ncz.gif|left|200px]] | [[Image:1ncz.gif|left|200px]] | ||
'''TROPONIN C''' | {{Structure | ||
|PDB= 1ncz |SIZE=350|CAPTION= <scene name='initialview01'>1ncz</scene>, resolution 1.8Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=TB:TERBIUM(III)+ION'>TB</scene> and <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''TROPONIN C''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1NCZ is a [ | 1NCZ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NCZ OCA]. | ||
==Reference== | ==Reference== | ||
X-ray structures of Mn, Cd and Tb metal complexes of troponin C., Rao ST, Satyshur KA, Greaser ML, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1996 Sep 1;52(Pt 5):916-22. PMID:[http:// | X-ray structures of Mn, Cd and Tb metal complexes of troponin C., Rao ST, Satyshur KA, Greaser ML, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1996 Sep 1;52(Pt 5):916-22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299599 15299599] | ||
[[Category: Gallus gallus]] | [[Category: Gallus gallus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: muscle protein]] | [[Category: muscle protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:55:13 2008'' | ||
Revision as of 10:55, 20 March 2008
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| 1ncz, resolution 1.8Å | |||||||||||||
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| Ligands: | TB and SO4 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
TROPONIN C
Overview
The crystal structures of three metal complexes of troponin C (TnC) have been determined and refined where the two occupied structural Ca(2+) sites in the C domain have been substituted by Mn(2+), Cd(2+) and Tb(3+). The X-ray intensity data were collected to 2.1, 1.8 and 1.8 A resolution, respectively, on the three metal complexes, which are isomorphous with Ca-TnC. The three complexes have r.m.s. deviations of 0.27, 0.25 and 0.35 A, respectively, for all protein atoms, from Ca-TnC. Irrespective of the charge on the metal (+2 or +3), the occupied sites 3 and 4 exhibit a distorted pentagonal bipyramidal coordination, like Ca-TnC, with seven ligands, six from the 12-residue binding loop and the seventh from a water molecule. Mn(2+) at site 4 seems to display a longer distance to one of the carboxyl bidentate ligands representing an intermediate coordination simulating the six-coordinate Mg(2+). The carboxyl O atoms of the bidentate Glu12 are displaced on the side of the equatorial plane passing through the remaining three ligands with one O atom closer to the plane (Delta of 0.11 to 0.76 A) than the other (Delta of 0.93 to 1.38 A). The two axial ligands are an aspartic carboxyl O atom and a water molecule. The metal is displaced (0.18 to 0.56 A) towards the water facing the water channel.
About this Structure
1NCZ is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
X-ray structures of Mn, Cd and Tb metal complexes of troponin C., Rao ST, Satyshur KA, Greaser ML, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1996 Sep 1;52(Pt 5):916-22. PMID:15299599
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