1njf: Difference between revisions
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[[Image:1njf.gif|left|200px]] | [[Image:1njf.gif|left|200px]] | ||
'''Nucleotide bound form of an isolated E. coli clamp loader gamma subunit''' | {{Structure | ||
|PDB= 1njf |SIZE=350|CAPTION= <scene name='initialview01'>1njf</scene>, resolution 2.30Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=ATG:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>ATG</scene> and <scene name='pdbligand=ADP:ADENOSINE-5'-DIPHOSPHATE'>ADP</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] | |||
|GENE= DnaX ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |||
}} | |||
'''Nucleotide bound form of an isolated E. coli clamp loader gamma subunit''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1NJF is a [ | 1NJF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NJF OCA]. | ||
==Reference== | ==Reference== | ||
Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit., Podobnik M, Weitze TF, O'Donnell M, Kuriyan J, Structure. 2003 Mar;11(3):253-63. PMID:[http:// | Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit., Podobnik M, Weitze TF, O'Donnell M, Kuriyan J, Structure. 2003 Mar;11(3):253-63. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12623013 12623013] | ||
[[Category: DNA-directed DNA polymerase]] | [[Category: DNA-directed DNA polymerase]] | ||
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
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[[Category: ATG]] | [[Category: ATG]] | ||
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: aaa+ atpase | [[Category: aaa+ atpase domain]] | ||
[[Category: protein-nucleotide complex]] | [[Category: protein-nucleotide complex]] | ||
[[Category: rossman-like fold]] | [[Category: rossman-like fold]] | ||
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[[Category: sensor 2]] | [[Category: sensor 2]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 12:57:41 2008'' | ||
Revision as of 10:57, 20 March 2008
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| 1njf, resolution 2.30Å | |||||||||||||
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| Ligands: | ZN, ATG and ADP | ||||||||||||
| Gene: | DnaX (Escherichia coli) | ||||||||||||
| Activity: | DNA-directed DNA polymerase, with EC number 2.7.7.7 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Nucleotide bound form of an isolated E. coli clamp loader gamma subunit
Overview
Sliding clamps are loaded onto DNA by ATP-driven clamp loader complexes. The structure of the E. coli clamp loader in a nucleotide-free state has been determined previously. We now report crystal structures of a truncated form of the isolated gamma-ATPase subunit, gamma(1-243), of the E. coli clamp loader, in nucleotide-free and bound forms. The gamma subunit adopts a defined conformation when empty, in which the nucleotide binding site is blocked. The binding of either ATPgammaS or ADP, which are shown to bind with equal affinity to gamma(1-243), induces a change in the relative orientation of the two domains such that nucleotides can be accommodated. This change would break one of the gamma:gamma interfaces seen in the empty clamp loader complex, and may represent one step in the activation process.
About this Structure
1NJF is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Nucleotide-induced conformational changes in an isolated Escherichia coli DNA polymerase III clamp loader subunit., Podobnik M, Weitze TF, O'Donnell M, Kuriyan J, Structure. 2003 Mar;11(3):253-63. PMID:12623013
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