3kxd: Difference between revisions

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[[Image:3kxd.png|left|200px]]
==Crystal structure of the mthk rck in complex with cadmium==
<StructureSection load='3kxd' size='340' side='right' caption='[[3kxd]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3kxd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus_str._delta_h Methanothermobacter thermautotrophicus str. delta h]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KXD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3KXD FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2aef|2aef]], [[2aej|2aej]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mthK, MTH_1520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=187420 Methanothermobacter thermautotrophicus str. Delta H])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3kxd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3kxd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3kxd RCSB], [http://www.ebi.ac.uk/pdbsum/3kxd PDBsum]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kx/3kxd_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
RCK is a cytoplasmic regulatory domain of calcium-gated potassium channels. Binding of Ca(2+) by RCK leads to channel activation through a series of yet unknown conformational changes. Structures of the K(+) channel, MthK, and its cytoplasmic RCK domain revealed two binding sites for Ca(2+) per dimer. We determined the crystal structure of RCK in complex with Cd(2+) at 2.2A resolution. Cd(2+) activates MthK more efficiently, and binds at the same binding sites for Ca(2+) but with reduced coordination number. Two additional binding sites for Cd(2+) are found per dimer; one on the main Rossman-fold lobe, and the other on the small lobe of RCK. Using patch-clamp experiments, we demonstrate that Cd(2+) binding to these novel sites enhances activation by Cd(2+) and not by Ca(2+). The structure reveals a large negatively charged surface patch in the proximity of the Ca(2+)/Cd(2+) binding sites, charge neutralization of which appears to promote the channel open state.


{{STRUCTURE_3kxd|  PDB=3kxd  |  SCENE=  }}
Structure of the MthK RCK in complex with cadmium.,Dvir H, Valera E, Choe S J Struct Biol. 2010 Aug;171(2):231-7. Epub 2010 Apr 4. PMID:20371380<ref>PMID:20371380</ref>


===Crystal structure of the mthk rck in complex with cadmium===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_20371380}}
 
==About this Structure==
[[3kxd]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus_str._delta_h Methanothermobacter thermautotrophicus str. delta h]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3KXD OCA].


==See Also==
==See Also==
*[[Potassium Channel|Potassium Channel]]
*[[Potassium Channel|Potassium Channel]]
 
== References ==
==Reference==
<references/>
<ref group="xtra">PMID:020371380</ref><references group="xtra"/>
__TOC__
</StructureSection>
[[Category: Methanothermobacter thermautotrophicus str. delta h]]
[[Category: Methanothermobacter thermautotrophicus str. delta h]]
[[Category: Choe, S.]]
[[Category: Choe, S]]
[[Category: Dvir, H.]]
[[Category: Dvir, H]]
[[Category: Alternative initiation]]
[[Category: Alternative initiation]]
[[Category: Cadmium]]
[[Category: Cadmium]]
Retrieved from "https://proteopedia.org/3kxd"