1nzs: Difference between revisions
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'''NMR structures of phosphorylated carboxy terminus of bovine rhodopsin in arrestin-bound state''' | {{Structure | ||
|PDB= 1nzs |SIZE=350|CAPTION= <scene name='initialview01'>1nzs</scene> | |||
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|LIGAND= | |||
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'''NMR structures of phosphorylated carboxy terminus of bovine rhodopsin in arrestin-bound state''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1NZS is a [ | 1NZS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NZS OCA]. | ||
==Reference== | ==Reference== | ||
The arrestin-bound conformation and dynamics of the phosphorylated carboxy-terminal region of rhodopsin., Kisselev OG, McDowell JH, Hargrave PA, FEBS Lett. 2004 Apr 30;564(3):307-11. PMID:[http:// | The arrestin-bound conformation and dynamics of the phosphorylated carboxy-terminal region of rhodopsin., Kisselev OG, McDowell JH, Hargrave PA, FEBS Lett. 2004 Apr 30;564(3):307-11. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15111114 15111114] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Hargrave, P A.]] | [[Category: Hargrave, P A.]] | ||
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[[Category: signal termination]] | [[Category: signal termination]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:03:55 2008'' | ||
Revision as of 11:03, 20 March 2008
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NMR structures of phosphorylated carboxy terminus of bovine rhodopsin in arrestin-bound state
Overview
Visual arrestin binds to the phosphorylated carboxy-terminal region of rhodopsin to block interactions with transducin and terminate signaling in the rod photoreceptor cells. A synthetic seven-phospho-peptide from the C-terminal region of rhodopsin, Rh(330-348), has been shown to bind arrestin and mimic inhibition of signal transduction. In this study, we examine conformational changes in this synthetic peptide upon binding to arrestin by high-resolution proton nuclear magnetic resonance (NMR). We show that the peptide is completely disordered in solution, but becomes structured upon binding to arrestin. A control, unphosphorylated peptide that fails to bind to arrestin remains highly disordered. Specific NMR distance constraints are used to model the arrestin-bound conformation. The models suggest that the phosphorylated carboxy-terminal region of rhodopsin, Rh(330-348), undergoes significant conformational changes and becomes structured upon binding to arrestin.
About this Structure
1NZS is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
The arrestin-bound conformation and dynamics of the phosphorylated carboxy-terminal region of rhodopsin., Kisselev OG, McDowell JH, Hargrave PA, FEBS Lett. 2004 Apr 30;564(3):307-11. PMID:15111114
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