1o7t: Difference between revisions
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[[Image:1o7t.gif|left|200px]] | [[Image:1o7t.gif|left|200px]] | ||
'''METAL NANOCLUSTERS BOUND TO THE FERRIC BINDING PROTEIN FROM NEISSERIA GONORRHOEAE.''' | {{Structure | ||
|PDB= 1o7t |SIZE=350|CAPTION= <scene name='initialview01'>1o7t</scene>, resolution 1.65Å | |||
|SITE= <scene name='pdbsite=HF1:Phf+Binding+Site+For+Chain+I'>HF1</scene> | |||
|LIGAND= <scene name='pdbligand=HF5:HF+OXO+CLUSTER+HF5'>HF5</scene>, <scene name='pdbligand=HF3:SMALLEST+HF-OXO-PHOSPHATE+CLUSTER+HF3'>HF3</scene> and <scene name='pdbligand=PHF:HF-OXO-PHOSPHATE CLUSTER PHF'>PHF</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''METAL NANOCLUSTERS BOUND TO THE FERRIC BINDING PROTEIN FROM NEISSERIA GONORRHOEAE.''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1O7T is a [ | 1O7T is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Neisseria_gonorrhoeae Neisseria gonorrhoeae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1O7T OCA]. | ||
==Reference== | ==Reference== | ||
A novel protein-mineral interface., Alexeev D, Zhu H, Guo M, Zhong W, Hunter DJ, Yang W, Campopiano DJ, Sadler PJ, Nat Struct Biol. 2003 Apr;10(4):297-302. PMID:[http:// | A novel protein-mineral interface., Alexeev D, Zhu H, Guo M, Zhong W, Hunter DJ, Yang W, Campopiano DJ, Sadler PJ, Nat Struct Biol. 2003 Apr;10(4):297-302. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12598891 12598891] | ||
[[Category: Neisseria gonorrhoeae]] | [[Category: Neisseria gonorrhoeae]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: periplasmic ferric binding protein]] | [[Category: periplasmic ferric binding protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:06:56 2008'' | ||
Revision as of 11:06, 20 March 2008
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| 1o7t, resolution 1.65Å | |||||||||||||
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| Sites: | HF1 | ||||||||||||
| Ligands: | HF5, HF3 and PHF | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
METAL NANOCLUSTERS BOUND TO THE FERRIC BINDING PROTEIN FROM NEISSERIA GONORRHOEAE.
Overview
Transferrins transport Fe3+ and other metal ions in mononuclear-binding sites. We present the first evidence that a member of the transferrin superfamily is able to recognize multi-nuclear oxo-metal clusters, small mineral fragments that are the most abundant forms of many metals in the environment. We show that the ferric ion-binding protein from Neisseria gonorrhoeae (nFbp) readily binds clusters of Fe3+, Ti4+, Zr4+ or Hf4+ in solution. The 1.7 A resolution crystal structure of Hf-nFbp reveals three distinct types of clusters in an open, positively charged cleft between two hinged protein domains. A di-tyrosyl cluster nucleation motif (Tyr195-Tyr196) is situated at the bottom of this cleft and binds either a trinuclear oxo-Hf cluster, which is capped by phosphate, or a pentanuclear cluster, which in turn can be capped with phosphate. This first high-resolution structure of a protein-mineral interface suggests a novel metal-uptake mechanism and provides a model for protein-mediated mineralization/dissimilation, which plays a critical role in geochemical processes.
About this Structure
1O7T is a Single protein structure of sequence from Neisseria gonorrhoeae. Full crystallographic information is available from OCA.
Reference
A novel protein-mineral interface., Alexeev D, Zhu H, Guo M, Zhong W, Hunter DJ, Yang W, Campopiano DJ, Sadler PJ, Nat Struct Biol. 2003 Apr;10(4):297-302. PMID:12598891
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