1qe4: Difference between revisions

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~~{{Theoretical_model}}~~

==BUILDING A REPLISOME FROM INTERACTING PIECES: SLIDING CLAMP COMPLEXED TO A PEPTIDE FROM DNA POLYMERASE AND A POLYMERASE EDITING COMPLEX==

== Structural highlights ==

<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QE4 FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qe4 FirstGlance], [http://www.ebi.ac.uk/pdbsum/1qe4 PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

We have solved the crystal structures of the bacteriophage RB69 sliding clamp, its complex with a peptide essential for DNA polymerase interactions, and the DNA polymerase complexed with primer-template DNA. The editing complex structure shows a partially melted duplex DNA exiting from the exonuclease domain at an unexpected angle and significant changes in the protein structure. The clamp complex shows the C-terminal 11 residues of polymerase bound in a hydrophobic pocket, and it allows docking of the editing and clamp structures together. The peptide binds to the sliding clamp at a position identical to that of a replication inhibitor peptide bound to PCNA, suggesting that the replication inhibitor protein p21CIP1 functions by competing with eukaryotic polymerases for the same binding pocket on the clamp.

~~[[Image~~:~~1qe4~~.~~png|left|200px]]~~

Building a replisome from interacting pieces: sliding clamp complexed to a peptide from DNA polymerase and a polymerase editing complex.,Shamoo Y, Steitz TA Cell. 1999 Oct 15;99(2):155-66. PMID:10535734<ref>PMID:10535734</ref>

~~{{STRUCTURE_1qe4| PDB=1qe4 | SCENE= }}~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~===BUILDING A REPLISOME FROM INTERACTING PIECES: SLIDING CLAMP COMPLEXED TO A PEPTIDE FROM DNA POLYMERASE AND A POLYMERASE EDITING COMPLEX===~~

== References ==

~~{{ABSTRACT_PUBMED_10535734}}~~

__TOC__

</StructureSection>

==~~Reference~~==

<~~ref group="xtra">PMID:010535734<~~/~~ref~~><~~references group="xtra"~~/>

[[Category: Shamoo, Y]]

[[Category: Steitz, T A]]

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@@ Line 1: / Line 1: @@
-{{Theoretical_model}}
+==BUILDING A REPLISOME FROM INTERACTING PIECES: SLIDING CLAMP COMPLEXED TO A PEPTIDE FROM DNA POLYMERASE AND A POLYMERASE EDITING COMPLEX==
+<StructureSection load='1qe4' size='340' side='right' caption='[[1qe4]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QE4 FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qe4 FirstGlance], [http://www.ebi.ac.uk/pdbsum/1qe4 PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+We have solved the crystal structures of the bacteriophage RB69 sliding clamp, its complex with a peptide essential for DNA polymerase interactions, and the DNA polymerase complexed with primer-template DNA. The editing complex structure shows a partially melted duplex DNA exiting from the exonuclease domain at an unexpected angle and significant changes in the protein structure. The clamp complex shows the C-terminal 11 residues of polymerase bound in a hydrophobic pocket, and it allows docking of the editing and clamp structures together. The peptide binds to the sliding clamp at a position identical to that of a replication inhibitor peptide bound to PCNA, suggesting that the replication inhibitor protein p21CIP1 functions by competing with eukaryotic polymerases for the same binding pocket on the clamp.
-[[Image:1qe4.png|left|200px]]
+Building a replisome from interacting pieces: sliding clamp complexed to a peptide from DNA polymerase and a polymerase editing complex.,Shamoo Y, Steitz TA Cell. 1999 Oct 15;99(2):155-66. PMID:10535734<ref>PMID:10535734</ref>
-{{STRUCTURE_1qe4|  PDB=1qe4  |  SCENE=  }}
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-===BUILDING A REPLISOME FROM INTERACTING PIECES: SLIDING CLAMP COMPLEXED TO A PEPTIDE FROM DNA POLYMERASE AND A POLYMERASE EDITING COMPLEX===
+== References ==
+<references/>
-{{ABSTRACT_PUBMED_10535734}}
+__TOC__
+</StructureSection>
-==Reference==
-<ref group="xtra">PMID:010535734</ref><references group="xtra"/>
 [[Category: Shamoo, Y]]
 [[Category: Steitz, T A]]