3cvz

Structural insights into the molecular organization of the S-layer from Clostridium difficile

spinqualitylabelsall models 3cvz, resolution 2.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Structural highlights 3cvz is a 4 chain structure with sequence from [clostridium_difficile [clostridium] difficile]. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: GLY, PO4 Gene: slpA ([Clostridium difficile]) Resources: FirstGlance, OCA, RCSB, PDBsum Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMed Clostridium difficile expresses a surface layer (S-layer) which coats the surface of the bacterium and acts as an adhesin facilitating interaction of the bacterium with host enteric cells. The S-layer contains a high-molecular-weight S-layer protein (HMW SLP) and its low-molecular-weight partner protein (LMW SLP). We show that these proteins form a tightly associated non-covalent complex, the H/L complex, and we identify the regions of both proteins responsible for complex formation. The 2.4 A X-ray crystal structure of a truncated derivative of the LMW SLP reveals two domains. Domain 1 has a two-layer sandwich architecture while domain 2, predicted to orientate towards the external environment, contains a novel fold. Small-angle X-ray scattering analysis of the H/L complex shows an elongated molecule, with the two SLPs arranged 'end-to-end' interacting with each other through a small contact area. Alignment of LMW SLPs, which exhibit high sequence diversity, reveals a core of conserved residues that could reflect functional conservation, while allowing for immune evasion through sequence variation. These structures are the first described for the S-layer of a bacterial pathogen, and provide insights into the assembly and biogenesis of the S-layer. Structural insights into the molecular organization of the S-layer from Clostridium difficile.,Fagan RP, Albesa-Jove D, Qazi O, Svergun DI, Brown KA, Fairweather NF Mol Microbiol. 2009 Mar;71(5):1308-22. Epub 2009 Jan 29. PMID:19183279 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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