1onj: Difference between revisions
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[[Image:1onj.gif|left|200px]] | [[Image:1onj.gif|left|200px]] | ||
'''Crystal structure of Atratoxin-b from Chinese cobra venom of Naja atra''' | {{Structure | ||
|PDB= 1onj |SIZE=350|CAPTION= <scene name='initialview01'>1onj</scene>, resolution 1.555Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''Crystal structure of Atratoxin-b from Chinese cobra venom of Naja atra''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1ONJ is a [ | 1ONJ is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Naja_atra Naja atra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ONJ OCA]. | ||
==Reference== | ==Reference== | ||
Purification, N-terminal sequencing, crystallization and preliminary structural determination of atratoxin-b, a short-chain alpha-neurotoxin from Naja atra venom., Lou X, Tu X, Pan G, Xu C, Fan R, Lu W, Deng W, Rao P, Teng M, Niu L, Acta Crystallogr D Biol Crystallogr. 2003 Jun;59(Pt 6):1038-42. Epub 2003, May 23. PMID:[http:// | Purification, N-terminal sequencing, crystallization and preliminary structural determination of atratoxin-b, a short-chain alpha-neurotoxin from Naja atra venom., Lou X, Tu X, Pan G, Xu C, Fan R, Lu W, Deng W, Rao P, Teng M, Niu L, Acta Crystallogr D Biol Crystallogr. 2003 Jun;59(Pt 6):1038-42. Epub 2003, May 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12777767 12777767] | ||
[[Category: Naja atra]] | [[Category: Naja atra]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: three-finger protein]] | [[Category: three-finger protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:13:12 2008'' | ||
Revision as of 11:13, 20 March 2008
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| 1onj, resolution 1.555Å | |||||||||||||
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| Ligands: | SO4 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Crystal structure of Atratoxin-b from Chinese cobra venom of Naja atra
Overview
Atratoxin-b, a short-chain alpha-neurotoxin purified from Naja atra (mainland Chinese cobra) venom using a three-step chromatography procedure, has an apparent molecular mass of 6950 Da with an alkaline pI value (>9.5) and consists of one single polypeptide chain as estimated by MALDI-TOF mass spectrometry and SDS-PAGE. The protein is toxic to mice, with an in vitro LD(50) of about 0.18 mg kg(-1). Its N-terminal amino-acid sequence, LECHNQQSSQTPTIT, displays a very high homology to those of other alpha-neurotoxins. The overall three-dimensional structure of atratoxin-b is very similar to that of the homologous erabutoxin-a, as shown by the crystallographic molecular replacement and preliminary refinement results, with an R factor and R(free) of 27 and 29%, respectively. The microcrystal slowly grew to dimensions of approximate 0.1 x 0.1 x 0.15 mm over eight months using hanging-drop vapour-diffusion method. It gave a set of diffraction data to 1.56 A resolution using X-rays of wavelength 1.1516 A generated by the X-ray Diffraction and Scattering Station of beamline U7B at the National Synchrotron Radiation Laboratory (Hefei, China); this is the first example of the use of this beamline in protein crystallography. The crystals belong to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = 49.28, c = 44.80 A, corresponding to one molecule per asymmetric unit and a volume-to-mass ratio of 1.96 A(3) Da(-1).
About this Structure
1ONJ is a Single protein structure of sequence from Naja atra. Full crystallographic information is available from OCA.
Reference
Purification, N-terminal sequencing, crystallization and preliminary structural determination of atratoxin-b, a short-chain alpha-neurotoxin from Naja atra venom., Lou X, Tu X, Pan G, Xu C, Fan R, Lu W, Deng W, Rao P, Teng M, Niu L, Acta Crystallogr D Biol Crystallogr. 2003 Jun;59(Pt 6):1038-42. Epub 2003, May 23. PMID:12777767
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