1ax3: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| Line 5: | Line 5: | ||
==Overview== | ==Overview== | ||
The high-resolution solution structure of the phosphocarrier protein, IIAglc from Bacillus subtilis is determined using 3D and 4D heteronuclear, NMR methods. B. subtilis IIAglc contains 162 amino acid residues and is, one of the larger proteins for which high-resolution solution structure, has been determined by NMR methods. The structures have been calculated, from a total of 2,232 conformational constraints. Comparison with the, X-ray crystal structure indicates that the overall fold is the same in, solution and in crystalline environments, although some local structural, differences are observed. These occur largely in turns and loops, and, mostly correspond to regions with high-temperature factors in the crystal, structure. The N-terminus of IIAglc is disordered in solution. The . | The high-resolution solution structure of the phosphocarrier protein, IIAglc from Bacillus subtilis is determined using 3D and 4D heteronuclear, NMR methods. B. subtilis IIAglc contains 162 amino acid residues and is, one of the larger proteins for which high-resolution solution structure, has been determined by NMR methods. The structures have been calculated, from a total of 2,232 conformational constraints. Comparison with the, X-ray crystal structure indicates that the overall fold is the same in, solution and in crystalline environments, although some local structural, differences are observed. These occur largely in turns and loops, and, mostly correspond to regions with high-temperature factors in the crystal, structure. The N-terminus of IIAglc is disordered in solution. The active, site is located in a concave region of the protein surface. The histidine, which accepts the phosphoryl group (His 83), interacts with a neighboring, histidine (His 68) and is surrounded by hydrophobic residues. | ||
==About this Structure== | ==About this Structure== | ||
1AX3 is a | 1AX3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Active as [http://en.wikipedia.org/wiki/Protein-N(pi)-phosphohistidine--sugar_phosphotransferase Protein-N(pi)-phosphohistidine--sugar phosphotransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.69 2.7.1.69] Structure known Active Site: PON. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AX3 OCA]. | ||
==Reference== | ==Reference== | ||
| Line 26: | Line 26: | ||
[[Category: transmembrane]] | [[Category: transmembrane]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 12:39:33 2007'' | ||
