Resolution: Difference between revisions
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The images at right were taken from a movie<ref name='movie'>The movie ([[Image:Resolution_holton.mpeg]]) was created by James Holton at the Advanced Light Source of the Berkeley Laboratory at the University of California. Holton kindly gave explicit permission to use this movie in Proteopedia. The original source was http://ucxray.berkeley.edu/~jamesh/movies.</ref> in which the atomic model and electron density map rock back and forth while the resolution value (uncertainty) increases from 0.5 to 5.0 Å. | The images at right were taken from a movie<ref name='movie'>The movie ([[Image:Resolution_holton.mpeg]]) was created by James Holton at the Advanced Light Source of the Berkeley Laboratory at the University of California. Holton kindly gave explicit permission to use this movie in Proteopedia. The original source was http://ucxray.berkeley.edu/~jamesh/movies.</ref> in which the atomic model and electron density map rock back and forth while the resolution value (uncertainty) increases from 0.5 to 5.0 Å. | ||
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[http://proteopedia.org/wiki/images/1/1a/Resolution_holton.mpeg '''PLAY MOVIE'''] | |||
</big>(Movie by James Holton at the Advanced Light Source of the Berkeley Laboratory at the University of California<ref name='movie' />.)</center> | |||
<!--COMMENTED OUT BY ERIC MARTZ MAY 15, 2014: I'M NOT SURE WHAT THIS TABLE WAS SUPPOSED TO ACCOMPLISH BUT IT BROKE THE LINK TO THE MOVIE! | |||
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| valign="top" |<swf width="300" height="200">http://www.proteopedia.org/wiki/images/e/e7/Resolutionholton.swf</swf> | | valign="top" |<swf width="300" height="200">http://www.proteopedia.org/wiki/images/e/e7/Resolutionholton.swf</swf> | ||
<br />Movie by James Holton at the Advanced Light Source of the Berkeley Laboratory at the University of California<ref name='movie' />. | |||
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At 0.5 Å in the movie, every atom<ref name="stills" /> of the tryptophan sidechain in the top center of the frame is clearly represented by a sphere of electron density. At 2.5 Å (a bit worse than the median in the [[PDB]]), the overall shape and position of the Trp sidechain is still clear, as is the alpha helical conformation of the main chain. However, at 5.0 Å, only an ill-fitting bump is present to signal the bulky Trp sidechain, and the alpha helix becomes a cylinder of electron density, from which the handedness of the helix may not be discernable. | At 0.5 Å in the movie, every atom<ref name="stills" /> of the tryptophan sidechain in the top center of the frame is clearly represented by a sphere of electron density. At 2.5 Å (a bit worse than the median in the [[PDB]]), the overall shape and position of the Trp sidechain is still clear, as is the alpha helical conformation of the main chain. However, at 5.0 Å, only an ill-fitting bump is present to signal the bulky Trp sidechain, and the alpha helix becomes a cylinder of electron density, from which the handedness of the helix may not be discernable. | ||
