4gwn: Difference between revisions
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[[ | ==Crystal structure of human mature meprin beta== | ||
<StructureSection load='4gwn' size='340' side='right' caption='[[4gwn]], [[Resolution|resolution]] 3.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4gwn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GWN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GWN FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4gwm|4gwm]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MEP1B ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Meprin_B Meprin B], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.63 3.4.24.63] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gwn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gwn RCSB], [http://www.ebi.ac.uk/pdbsum/4gwn PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ectodomain shedding at the cell surface is a major mechanism to regulate the extracellular and circulatory concentration or the activities of signaling proteins at the plasma membrane. Human meprin beta is a 145-kDa disulfide-linked homodimeric multidomain type-I membrane metallopeptidase that sheds membrane-bound cytokines and growth factors, thereby contributing to inflammatory diseases, angiogenesis, and tumor progression. In addition, it cleaves amyloid precursor protein (APP) at the beta-secretase site, giving rise to amyloidogenic peptides. We have solved the X-ray crystal structure of a major fragment of the meprin beta ectoprotein, the first of a multidomain oligomeric transmembrane sheddase, and of its zymogen. The meprin beta dimer displays a compact shape, whose catalytic domain undergoes major rearrangement upon activation, and reveals an exosite and a sugar-rich channel, both of which possibly engage in substrate binding. A plausible structure-derived working mechanism suggests that substrates such as APP are shed close to the plasma membrane surface following an "N-like" chain trace. | |||
Structural basis for the sheddase function of human meprin beta metalloproteinase at the plasma membrane.,Arolas JL, Broder C, Jefferson T, Guevara T, Sterchi EE, Bode W, Stocker W, Becker-Pauly C, Gomis-Ruth FX Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):16131-6. doi:, 10.1073/pnas.1211076109. Epub 2012 Sep 17. PMID:22988105<ref>PMID:22988105</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Meprin B]] | [[Category: Meprin B]] | ||
[[Category: Arolas, J L | [[Category: Arolas, J L]] | ||
[[Category: Becker-Pauly, C | [[Category: Becker-Pauly, C]] | ||
[[Category: Bode, W | [[Category: Bode, W]] | ||
[[Category: Broder, C | [[Category: Broder, C]] | ||
[[Category: Gomis-Ruth, F X | [[Category: Gomis-Ruth, F X]] | ||
[[Category: Guevara, T | [[Category: Guevara, T]] | ||
[[Category: Jefferson, T | [[Category: Jefferson, T]] | ||
[[Category: Sterchi, E E | [[Category: Sterchi, E E]] | ||
[[Category: Stocker, W | [[Category: Stocker, W]] | ||
[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Multidomain structure]] | [[Category: Multidomain structure]] | ||
Revision as of 09:15, 10 December 2014
Crystal structure of human mature meprin beta
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