Sandbox Reserved 640: Difference between revisions
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This heme prosthetic group consists of four 5-membered pyrrole rings, forming a cyclic ring around a central iron (Fe) atam. This iron atom is contained within four equatorial nitrogens, in addition to another nitrogen from a close histidine residue and an opposite dioxygen (Ref 9). More specifically, the ligand contact residues are Phe30, His63, and Val67 (Ref 7). ***Add a section about H-bonding of the ligand with the Lb and how the heme group fits into the molecule*** | This heme prosthetic group consists of four 5-membered pyrrole rings, forming a cyclic ring around a central iron (Fe) atam. This iron atom is contained within four equatorial nitrogens, in addition to another nitrogen from a close histidine residue and an opposite dioxygen (Ref 9). More specifically, the ligand contact residues are Phe30, His63, and Val67 (Ref 7). ***Add a section about H-bonding of the ligand with the Lb and how the heme group fits into the molecule*** | ||
The methods by which various | The methods by which various Leghemoglobins were purified, and then analyzed, are as follows: | ||
-Ammonium Sulfate Precipitation | |||
-Concentration by Ultrafiltration | |||
-Electrophoresis | |||
-Anion-exchange Column Chromatography | |||
-Isoelectrofocusing | |||
-Nuclear Magnetic Radiance (NMR) | |||
-X-ray crystallography (Ref 1 and 6) | |||
