1ppg: Difference between revisions
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[[Image:1ppg.gif|left|200px]] | [[Image:1ppg.gif|left|200px]] | ||
'''THE REFINED 2.3 ANGSTROMS CRYSTAL STRUCTURE OF HUMAN LEUKOCYTE ELASTASE IN A COMPLEX WITH A VALINE CHLOROMETHYL KETONE INHIBITOR''' | {{Structure | ||
|PDB= 1ppg |SIZE=350|CAPTION= <scene name='initialview01'>1ppg</scene>, resolution 2.3Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/Leukocyte_elastase Leukocyte elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.37 3.4.21.37] | |||
|GENE= | |||
}} | |||
'''THE REFINED 2.3 ANGSTROMS CRYSTAL STRUCTURE OF HUMAN LEUKOCYTE ELASTASE IN A COMPLEX WITH A VALINE CHLOROMETHYL KETONE INHIBITOR''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1PPG is a [ | 1PPG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PPG OCA]. | ||
==Reference== | ==Reference== | ||
The refined 2.3 A crystal structure of human leukocyte elastase in a complex with a valine chloromethyl ketone inhibitor., Wei AZ, Mayr I, Bode W, FEBS Lett. 1988 Jul 18;234(2):367-73. PMID:[http:// | The refined 2.3 A crystal structure of human leukocyte elastase in a complex with a valine chloromethyl ketone inhibitor., Wei AZ, Mayr I, Bode W, FEBS Lett. 1988 Jul 18;234(2):367-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/3391280 3391280] | ||
[[Category: Leukocyte elastase]] | [[Category: Leukocyte elastase]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: hydrolase(serine proteinase)]] | [[Category: hydrolase(serine proteinase)]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:27:18 2008'' | ||
Revision as of 11:27, 20 March 2008
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| 1ppg, resolution 2.3Å | |||||||||||||
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| Activity: | Leukocyte elastase, with EC number 3.4.21.37 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
THE REFINED 2.3 ANGSTROMS CRYSTAL STRUCTURE OF HUMAN LEUKOCYTE ELASTASE IN A COMPLEX WITH A VALINE CHLOROMETHYL KETONE INHIBITOR
Overview
The stoichiometric complex formed between human leukocyte elastase and a synthetic MeO-Suc-Ala-Ala-Pro-Val chloromethyl ketone inhibitor was co-crystallized and its X-ray structure determined, using Patterson search methods. Its structure has been crystallographically refined to a final R value of 0.145 (8.0 and 2.3 A). The enzyme structure is very similar to that recently observed in a complex formed with the ovomucoid third domain from turkey [(1986) EMBO J. 5,2453-2458]. The rms deviation of all alpha-carbon atoms is 0.32 A. The peptidic inhibitor is bound in a similar overall conformation as the ovomucoid binding segment. Covalent bonds are formed between Val-P1 of the inhibitor and His-57 NE2 and Ser-195 OG of the enzyme. The carbonyl carbon is tetrahedrally deformed to a hemiketal. The valine side chain is arranged in the S1 pocket in the g-conformation.
Disease
Known diseases associated with this structure: Hematopoiesis, cyclic OMIM:[130130], Neutropenia, congenital OMIM:[130130]
About this Structure
1PPG is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
The refined 2.3 A crystal structure of human leukocyte elastase in a complex with a valine chloromethyl ketone inhibitor., Wei AZ, Mayr I, Bode W, FEBS Lett. 1988 Jul 18;234(2):367-73. PMID:3391280
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