Lambda repressor: Difference between revisions
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==C-Terminal Domain (CTD)== | ==C-Terminal Domain (CTD)== | ||
The CTD of Lambda Repressor assumes a structural conformation similar to a knotted β-sheet and is composed of 104 amino acid resides (residues 132-236). This conformation is key in establishing the dimer-forming interaction with the CTD of the opposite chain. The CTD also facilitates the dimer-dimer interaction necessary for cooperative-binding repression. The active site of the auto-cleavage mechanism of Lambda Repressor is on the CTD. Two amino acid residues mediate the auto-cleavage activity of the repressor, Lys192 and Ser149 (). | The CTD of Lambda Repressor assumes a structural conformation similar to a knotted β-sheet and is composed of 104 amino acid resides (residues 132-236). This conformation is key in establishing the dimer-forming interaction with the CTD of the opposite chain. The CTD also facilitates the dimer-dimer interaction necessary for cooperative-binding repression. The active site of the auto-cleavage mechanism of Lambda Repressor is on the CTD. Two amino acid residues mediate the auto-cleavage activity of the repressor, Lys192 and Ser149 (). <StructureSection load='3bdn' size='350' side='left' caption=' The auto-cleavage active site on the CTD is highlighted in yellow (residues 149, 192). (PDB entry [[3bdn]])' scene='Bacteriophage_Lambda_Repressor_cI/Ctd-_auto-cleavage_active_site/3'></StructureSection> | ||
<StructureSection load='3bdn' size='350' side='left' caption='CTD of Lambda Repressor cI (PDB entry [[3bdn]])' scene=''></StructureSection> | <StructureSection load='3bdn' size='350' side='left' caption='CTD of Lambda Repressor cI (PDB entry [[3bdn]])' scene=''></StructureSection> | ||
==Connecting Region== | ==Connecting Region== | ||
The region connecting the NTD to the CTD consists of 38 amino acid residues and contains the CSR. The CSR is a long loop (residues 106-126) and serves to stabilize the dimer through interactions with the CSR of the opposite chain. Cleavage occurs between Ala111 and Gly 112 in the CSR. <StructureSection load='3bdn' size='350' side=' | The region connecting the NTD to the CTD consists of 38 amino acid residues and contains the CSR. The CSR is a long loop (residues 106-126) and serves to stabilize the dimer through interactions with the CSR of the opposite chain. Cleavage occurs between Ala111 and Gly 112 in the CSR. | ||
<StructureSection load='3bdn' size='350' side='right' caption='The cleavage site of the connecting region is highlighted in red (residues 111-112), while the auto-cleavage active site of the CTD is highlighted in yellow(residues 149, 192). (PDB entry [[3bdn]])' scene='Bacteriophage_Lambda_Repressor_cI/Cleavage_site_and_active_site/1'></StructureSection> | |||
==N-Terminal Domain (NTD)== | |||
==Reference== | ==Reference== | ||
<ref group="xtra">PMID:18432246</ref><references group="xtra"/> | <ref group="xtra">PMID:18432246</ref><references group="xtra"/> | ||
<ref group="xtra">PMID:1387915</ref><references group="xtra"/> | <ref group="xtra">PMID:1387915</ref><references group="xtra"/> | ||
