1pxe: Difference between revisions
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[[Image:1pxe.gif|left|200px]]< | [[Image:1pxe.gif|left|200px]] | ||
'''Solution Structure of a CCHHC Domain of Neural Zinc Finger Factor-1''' | {{Structure | ||
|PDB= 1pxe |SIZE=350|CAPTION= <scene name='initialview01'>1pxe</scene> | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |||
|ACTIVITY= | |||
|GENE= NZF-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus]) | |||
}} | |||
'''Solution Structure of a CCHHC Domain of Neural Zinc Finger Factor-1''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1PXE is a [ | 1PXE is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PXE OCA]. | ||
==Reference== | ==Reference== | ||
Solution structure of a CCHHC domain of neural zinc finger factor-1 and its implications for DNA binding., Berkovits-Cymet HJ, Amann BT, Berg JM, Biochemistry. 2004 Feb 3;43(4):898-903. PMID:[http:// | Solution structure of a CCHHC domain of neural zinc finger factor-1 and its implications for DNA binding., Berkovits-Cymet HJ, Amann BT, Berg JM, Biochemistry. 2004 Feb 3;43(4):898-903. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14744132 14744132] | ||
[[Category: Rattus norvegicus]] | [[Category: Rattus norvegicus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: neural zinc finger factor-1]] | [[Category: neural zinc finger factor-1]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:30:10 2008'' | ||
Revision as of 11:30, 20 March 2008
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| 1pxe | |||||||||||||
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| Ligands: | ZN | ||||||||||||
| Gene: | NZF-1 (Rattus norvegicus) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
Solution Structure of a CCHHC Domain of Neural Zinc Finger Factor-1
Overview
The structure of a CCHHC zinc-binding domain from neural zinc finger factor-1 (NZF-1) has been determined in solution though the use of NMR methods. This domain is a member of a family of domains that have the Cys-X(4)-Cys-X(4)-His-X(7)-His-X(5)-Cys consensus sequence. The structure determination reveals a novel fold based around a zinc(II) ion coordinated to three Cys residues and the second of the two conserved His residues. The other His residue is stacked between the metal-coordinated His residue and a relatively conserved aromatic residue. Analysis of His to Gln sequence variants reveals that both His residues are required for the formation of a well-defined structure, but neither is required for high-affinity metal binding at a tetrahedral site. The structure suggests that a two-domain protein fragment and a double-stranded DNA binding site may interact with a common two-fold axis relating the two domains and the two half-sites of the DNA-inverted repeat.
About this Structure
1PXE is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Solution structure of a CCHHC domain of neural zinc finger factor-1 and its implications for DNA binding., Berkovits-Cymet HJ, Amann BT, Berg JM, Biochemistry. 2004 Feb 3;43(4):898-903. PMID:14744132
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