Resolvase: Difference between revisions
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{{STRUCTURE_1kcf| PDB=1kcf | SIZE=300| SCENE=Resolvase/Cv/1 |right|CAPTION=Yeast Holliday junction resolvase dimer complex with sulfate, [[1kcf]] }} | {{STRUCTURE_1kcf| PDB=1kcf | SIZE=300| SCENE=Resolvase/Cv/1 |right|CAPTION=Yeast Holliday junction resolvase dimer complex with sulfate, [[1kcf]] }} | ||
[[Resolvase]] or '''recombinase''' (Rec) is a nuclease which is involved in DNA recombination. The Holliday junction resolvase (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. Tyr-recombinases include Cre, FLP and lambda integrase (LamInt). Ser-recombinases (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase. RadA recombinase promotes DNA recombination. The images at the left and at the right correspond to one representative resolvase structure, ''i.e.'' the crystal structure of Ydc2 (also known as SpCce1), a Holliday junction resolvase from the fission yeast ''Schizosaccharomyces pombe'' that is involved in the maintenance of mitochondrial DNA ([[1kcf]]). Two sulfate ions are shown as space filling objects. Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:11726496</ref>. | [[Resolvase]] or '''recombinase''' (Rec) is a nuclease which is involved in DNA recombination. The Holliday junction resolvase (HJR) resolves 4-way DNA intermediates known as Holliday junctions. Recombination of 2 DNA sites occurs when recombinase binds to the 2 strands. According to the binding residue, the recombinases are grouped to Tyr- and Ser-recombinase. Tyr-recombinases include Cre, FLP and lambda integrase (LamInt). Ser-recombinases (S-rec) include gamma-delta resolvase (GDR), Tn3 resolvase and phiC31 integrase. RadA recombinase promotes DNA recombination. The images at the left and at the right correspond to one representative resolvase structure, ''i.e.'' the crystal structure of Ydc2 (also known as SpCce1), a Holliday junction resolvase from the fission yeast ''Schizosaccharomyces pombe'' that is involved in the maintenance of mitochondrial DNA ([[1kcf]]). Two sulfate ions are shown as space filling objects. Detailed analysis of its <scene name='Resolvase/Cv/2'>dimeric structure</scene> suggests mechanisms for junction isomerization and communication between the two active sites <ref>PMID:11726496</ref>. | ||
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==3D structures of resolvase== | ==3D structures of resolvase== | ||
