Sandbox Reserved 646

This Sandbox is Reserved from 30/08/2012, through 01/02/2013 for use in the course "Proteins and Molecular Mechanisms" taught by Robert B. Rose at the North Carolina State University, Raleigh, NC USA. This reservation includes Sandbox Reserved 636 through Sandbox Reserved 685.

To get started: Click the edit this page tab at the top. Save the page after each step, then edit it again. Click the 3D button (when editing, above the wikitext box) to insert Jmol. show the Scene authoring tools, create a molecular scene, and save it. Copy the green link into the page. Add a description of your scene. Use the buttons above the wikitext box for bold, italics, links, headlines, etc. More help: Help:Editing For more help, look at this link: https://proteopedia.org/w/Help:Getting_Started_in_Proteopedia Arginase spinqualitylabelsall models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image   Print3D Arginase is an enzyme found within the important urea cycle. Arginase is the final enzyme that allows the Urea cycle to complete its fifth and last step. Arginase takes on two distinct forms: Class I and II. The grouping into classes refers to the catalytic activity that the enzyme is capable of performing. In addition, each class are encoded by a different gene. Arginase I (arg1) is found in the cytoplasm of liver cells functioning in the urea cycle, while arginase II (arg2) is found in the mitochondria and functions in the kidney. In interest of our studies, we will refer to arginase I as arginase. Arginase belongs to the enzymatic class hydrolase. Arginase functions by cleaving the amino acid arginine with water to produce urea. When one studies enzymes, he pays careful attention the it’s specific activity. Specific activity quantifies the amount of product formed by the enzyme in reference to a given time per concentration of a protein. Arginase is known to have high specific activity and functions to produce a good amount of urea. In addition, this enzyme allows the regeneration of ornithine which is the final product of the urea cycle. When there is a deficiency in the arg1 gene it is understood that one is undergoing an urea cycle disorder. In people with arginase deficiency, arginase is either missing or harmed, therefore arginine is not metabolized correctly. In lieu of this, it is impossible for urea to be formed, leaving the excess nitrogen to accumulate in the blood in the form of ammonia. Which leads to serious problems in the body. Mechanism Arginine + H2O → Ornithine + Urea The mechanism regarding arginase is highly important in the production of urea through the urea cycle. During the final step of the urea cycle, the amino acid arginine is present and needs to be cleaved in order for urea to be produced. Arginine is hydrolyzed and cleaved with the hydroxyl at the end of the amino group. We refer to this group as a guanidium end. The guanidium group is positively charged and has a high pKa. Therefore, it is willing to donate a proton since it has a hydrogen to give. First, the ligand will ionize the water to form hydroxide. The hydroxide now attacks the guanidine carbon and protons are transferred from the hydroxide to the substrate bridging. In the third and final step protons are transferred through a proton shuffle to create ornithine and urea. Afterwards, the urea is excreted through urine. Leaving the excess ornithine to be recycled through the final step in the cycle to react with citrulline and eliminate ammonia from the body. Structure Isomers Topology Medical Application File:AntibodARG.jpg The urea cycle is important for removing urea from the body so that the body is able to regulate and carry on its normal functions. However, if the body is not able to get dispose of urea, it spells trouble for specific bodily functions. Currently, we understand that arginase functions to break down the amino acid arginine into ornithine while releasing urea. However, we know that the deficiency of arginase is strictly inherited and thereby a genetic disorder. When argininase is deficient, it causes a phenomenon of a build up in ammonia and arginine. An accumulation of high levels of ammonia can be toxic to the body and causes neurological effects. Symptoms Spastic tetraplegia Progressive mental retardation Seizures Hyperactivity Growth failure Elevated blood ammonia level Enlarged liver Lack of appetite Vomiting Increased blood level of arginine Muscle stiffness Spasticity Tremor Balance problems Coordination problems Irritability