Sandbox Reserved 654: Difference between revisions

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= PCAF =
= p300/CBP-associated factor =
<Structure load='1JM4' size='500' frame='true' align='right' caption='This is a model of the pheylalanine hydroxylase dimer as found in humans. The green ball in within each subunit represents the iron ion in the catalytic domains.' scene='Insert optional scene name here' />
<Structure load='1JM4' size='500' frame='true' align='right' caption='This is a model of the pheylalanine hydroxylase dimer as found in humans. The green ball in within each subunit represents the iron ion in the catalytic domains.' scene='Insert optional scene name here' />


'''PCAF'''(PCAF), Introduction stuff.<ref> sample ref </ref>  
'''p300/CBP-associated factor''' (PCAF), Introduction stuff.<ref> sample ref </ref>  


== '''Structure''' ==
== '''Structure''' ==
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== '''Mechanism''' ==
== '''Mechanism''' ==
Although the exact mechanism of phenylalanine degradation is still not fully understood, the main reaction requires the addition of an hydroxyl group to the benzene ring of the phenylalanine residue. In order for this process to occur, the cofactor tetrahydrobiopterin(BH4) loses two hydrogen atoms to become dihydrobiopterin. BH4 acts as a reductant by reducing one of the diatomic oxygens while the other is added to the 6-membered ring. In order to stabilize the substrate- enzyme complex as this reaction occurs, an iron atom within the protein is necessary. It is within the active site that the hydrogen atom from phenylalanine is stripped off and replaced with a hydroxyl group.<ref> College, Davidson. Phenylalanine Hydroxylase [http://www.bio.davidson.edu/Courses/Molbio/MolStudents/spring2010/Piper/Protein.html] </ref>   
While the detailed molecular mechanisms underlying Tat dissociation from TAR RNA and its transactivation of transcription of the integrated HIV-1 genome remain elusive, increasing lines of evidence suggest that Tat activity requires its association with several multiprotein complexes, which include histone acetyltransferase (HAT) and p300/CBP-associated factor (PCAF)<ref> http://www.sciencedirect.com/science/article/pii/S1097276502004835 </ref>   
[[Image:Phenylalanine_Hydroxylase_mechanism.jpg |thumb|200 px|center|Reaction catalyzed by PheOH]]
[[Image:Phenylalanine_Hydroxylase_mechanism.jpg |thumb|200 px|center|Reaction catalyzed by PheOH]]


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