1qfx: Difference between revisions
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[[Image:1qfx.jpg|left|200px]] | [[Image:1qfx.jpg|left|200px]] | ||
'''PH 2.5 ACID PHOSPHATASE FROM ASPERGILLUS NIGER''' | {{Structure | ||
|PDB= 1qfx |SIZE=350|CAPTION= <scene name='initialview01'>1qfx</scene>, resolution 2.4Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> and <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | |||
|ACTIVITY= [http://en.wikipedia.org/wiki/3-phytase 3-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.8 3.1.3.8] | |||
|GENE= | |||
}} | |||
'''PH 2.5 ACID PHOSPHATASE FROM ASPERGILLUS NIGER''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1QFX is a [ | 1QFX is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QFX OCA]. | ||
==Reference== | ==Reference== | ||
Crystal structure of Aspergillus niger pH 2.5 acid phosphatase at 2. 4 A resolution., Kostrewa D, Wyss M, D'Arcy A, van Loon AP, J Mol Biol. 1999 May 21;288(5):965-74. PMID:[http:// | Crystal structure of Aspergillus niger pH 2.5 acid phosphatase at 2. 4 A resolution., Kostrewa D, Wyss M, D'Arcy A, van Loon AP, J Mol Biol. 1999 May 21;288(5):965-74. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10329192 10329192] | ||
[[Category: 3-phytase]] | [[Category: 3-phytase]] | ||
[[Category: Aspergillus niger]] | [[Category: Aspergillus niger]] | ||
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[[Category: phosphomonoesterase]] | [[Category: phosphomonoesterase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:36:54 2008'' | ||
Revision as of 11:36, 20 March 2008
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| 1qfx, resolution 2.4Å | |||||||||||||
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| Ligands: | NAG, SO4 and GOL | ||||||||||||
| Activity: | 3-phytase, with EC number 3.1.3.8 | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
PH 2.5 ACID PHOSPHATASE FROM ASPERGILLUS NIGER
Overview
The crystal structure of Aspergillus niger pH 2.5 acid phosphatase (EC 3.1.3.2) has been determined at 2.4 A resolution. In the crystal, two dimers form a tetramer in which the active sites are easily accessible to substrates. The main contacts in the dimer come from the N termini, each lying on the surface of the neighbouring molecule. The monomer consists of two domains, with the active site located at their interface. The active site has a highly conserved catalytic center and a charge distribution, which explains the highly acidic pH optimum and the broad substrate specificity of the enzyme.
About this Structure
1QFX is a Single protein structure of sequence from Aspergillus niger. Full crystallographic information is available from OCA.
Reference
Crystal structure of Aspergillus niger pH 2.5 acid phosphatase at 2. 4 A resolution., Kostrewa D, Wyss M, D'Arcy A, van Loon AP, J Mol Biol. 1999 May 21;288(5):965-74. PMID:10329192
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