1bys: Difference between revisions
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==Overview== | ==Overview== | ||
The first crystal structure of a phospholipase D (PLD) family member has, been determined at 2.0 A resolution. The PLD superfamily is defined by a, common sequence motif, HxK(x)4D(x)6GSxN, and includes enzymes involved in, signal transduction, lipid biosynthesis, endonucleases and open reading, frames in pathogenic viruses and bacteria. The crystal structure suggests, that residues from two sequence motifs form a single active site. A, histidine residue from one motif acts as a nucleophile in the catalytic, mechanism, forming a phosphoenzyme intermediate, whereas a histidine, residue from the other motif appears to function as a general acid in the, cleavage of the phosphodiester bond. The structure suggests that the, conserved lysine residues are involved in phosphate binding. .. | The first crystal structure of a phospholipase D (PLD) family member has, been determined at 2.0 A resolution. The PLD superfamily is defined by a, common sequence motif, HxK(x)4D(x)6GSxN, and includes enzymes involved in, signal transduction, lipid biosynthesis, endonucleases and open reading, frames in pathogenic viruses and bacteria. The crystal structure suggests, that residues from two sequence motifs form a single active site. A, histidine residue from one motif acts as a nucleophile in the catalytic, mechanism, forming a phosphoenzyme intermediate, whereas a histidine, residue from the other motif appears to function as a general acid in the, cleavage of the phosphodiester bond. The structure suggests that the, conserved lysine residues are involved in phosphate binding. Large-scale, genomic sequencing revealed that there are many PLD family members. Our, results suggest that all of these proteins may possess a common structure, and catalytic mechanism. | ||
==About this Structure== | ==About this Structure== | ||
1BYS is a | 1BYS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium] with WO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: ACT. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BYS OCA]. | ||
==Reference== | ==Reference== | ||
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[[Category: phosphodiesterase]] | [[Category: phosphodiesterase]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 14:24:01 2007'' | ||
