1i9f: Difference between revisions

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[[~~Image~~:1i9f.~~png|left|200px~~]]

==STRUCTURAL CHARACTERIZATION OF THE COMPLEX OF THE REV RESPONSE ELEMENT RNA WITH A SELECTED PEPTIDE==

== Structural highlights ==

<table><tr><td colspan='2'>[[1i9f]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I9F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I9F FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i9f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i9f RCSB], [http://www.ebi.ac.uk/pdbsum/1i9f PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

INTRODUCTION: The RSG-1.2 peptide was selected for specific binding to the Rev response element RNA, as the natural Rev peptide does. The RSG-1.2 sequence has features incompatible with the helical structure of the bound Rev peptide, indicating that it must bind in a different conformation. RESULTS: The binding of the RSG-1.2 peptide to the Rev response element RNA was characterized using multinuclear, multidimensional NMR. The RSG-1.2 peptide is shown to bind with the N-terminal segment of the peptide along the major groove in an extended conformation and turn preceding a C-terminal helical segment, which crosses the RNA groove in the region widened by the presence of purine-purine base pairs. These features make the details of the bound state rather different than that of the Rev peptide which targets the same RNA sequence binding as a single helix along the groove axis. CONCLUSIONS: These studies further demonstrate the versatility of arginine-rich peptides in recognition of specific RNA elements and the lack of conserved structural features in the bound state.

~~{{STRUCTURE_1i9f| PDB=1i9f | SCENE= }}~~

Structural characterization of the complex of the Rev response element RNA with a selected peptide.,Zhang Q, Harada K, Cho HS, Frankel AD, Wemmer DE Chem Biol. 2001 May;8(5):511-20. PMID:11358697<ref>PMID:11358697</ref>

~~===STRUCTURAL CHARACTERIZATION OF THE COMPLEX OF THE REV RESPONSE ELEMENT RNA WITH A SELECTED PEPTIDE===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_11358697}}~~

== References ==

==~~About this Structure~~==

__TOC__

~~[[1i9f]] is a 2 chain structure. Full experimental information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=1I9F OCA].~~

</StructureSection>

[[Category: Cho, H S.]]

[[Category: Frankel, A.]]

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-[[Image:1i9f.png|left|200px]]
+==STRUCTURAL CHARACTERIZATION OF THE COMPLEX OF THE REV RESPONSE ELEMENT RNA WITH A SELECTED PEPTIDE==
+<StructureSection load='1i9f' size='340' side='right' caption='[[1i9f]], [[NMR_Ensembles_of_Models | 7 NMR models]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1i9f]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I9F OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1I9F FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1i9f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i9f OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1i9f RCSB], [http://www.ebi.ac.uk/pdbsum/1i9f PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+INTRODUCTION: The RSG-1.2 peptide was selected for specific binding to the Rev response element RNA, as the natural Rev peptide does. The RSG-1.2 sequence has features incompatible with the helical structure of the bound Rev peptide, indicating that it must bind in a different conformation. RESULTS: The binding of the RSG-1.2 peptide to the Rev response element RNA was characterized using multinuclear, multidimensional NMR. The RSG-1.2 peptide is shown to bind with the N-terminal segment of the peptide along the major groove in an extended conformation and turn preceding a C-terminal helical segment, which crosses the RNA groove in the region widened by the presence of purine-purine base pairs. These features make the details of the bound state rather different than that of the Rev peptide which targets the same RNA sequence binding as a single helix along the groove axis. CONCLUSIONS: These studies further demonstrate the versatility of arginine-rich peptides in recognition of specific RNA elements and the lack of conserved structural features in the bound state.
-{{STRUCTURE_1i9f|  PDB=1i9f  |  SCENE=  }}
+Structural characterization of the complex of the Rev response element RNA with a selected peptide.,Zhang Q, Harada K, Cho HS, Frankel AD, Wemmer DE Chem Biol. 2001 May;8(5):511-20. PMID:11358697<ref>PMID:11358697</ref>
-===STRUCTURAL CHARACTERIZATION OF THE COMPLEX OF THE REV RESPONSE ELEMENT RNA WITH A SELECTED PEPTIDE===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_11358697}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[1i9f]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I9F OCA].
+</StructureSection>
 [[Category: Cho, H S.]]
 [[Category: Frankel, A.]]