1guc: Difference between revisions

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[[~~Image~~:1guc.~~png|left|200px~~]]

==SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM, SYMMETRIC GU MISMATCHES, 30 STRUCTURES==

== Structural highlights ==

<table><tr><td colspan='2'>[[1guc]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GUC FirstGlance]. <br>

</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1guc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1guc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1guc RCSB], [http://www.ebi.ac.uk/pdbsum/1guc PDBsum]</span></td></tr>

<table>

== Publication Abstract from PubMed ==

The duplex (rGAGGUCUC)2 contains the motif [sequence: see text] which is unusually stable compared with other symmetric tandem GU mismatches and occurs in the P5 helix of the group I intron of Tetrahymena thermophila. The three-dimensional solution structure of (rGAGGUCUC)2 was determined using two-dimensional NMR and a simulated annealing protocol. The structure is remarkably similar to the A-DNA crystal structure of (dGGGGTCCC)2 [Kneale, G., Brown, T., & Kennard, O. (1985) J. Mol. Biol. 186, 805-814] which contains the analogous motif [sequence: see text]. Incorporation of the [sequence: see text] motif has little effect on backbone torsion angles and helical parameters compared with standard A-form. The only significant departure from A-form is a slight overtwisting 5' of the G in the GU mismatch and a displacement of the mismatches toward the minor groove. Inspection of stacking patterns of this structure and comparison with symmetric tandem GT mismatches in A-DNA oligonucleotides from crystal structure data suggest that electrostatics are important in determining motif stability.

~~{{STRUCTURE_1guc| PDB=1guc | SCENE= }}~~

Investigation of the structural basis for thermodynamic stabilities of tandem GU mismatches: solution structure of (rGAGGUCUC)2 by two-dimensional NMR and simulated annealing.,McDowell JA, Turner DH Biochemistry. 1996 Nov 12;35(45):14077-89. PMID:8916893<ref>PMID:8916893</ref>

~~===SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM~~, ~~SYMMETRIC GU MISMATCHES, 30 STRUCTURES===~~

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

</div>

~~{{ABSTRACT_PUBMED_8916893}}~~

== References ==

==~~About this Structure~~==

__TOC__

~~[[1guc]] is a 2 chain structure. Full experimental information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=1GUC OCA].~~

</StructureSection>

[[Category: Mcdowell, J A.]]

[[Category: Turner, D H.]]

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-[[Image:1guc.png|left|200px]]
+==SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM, SYMMETRIC GU MISMATCHES, 30 STRUCTURES==
+<StructureSection load='1guc' size='340' side='right' caption='[[1guc]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[1guc]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1GUC FirstGlance]. <br>
+</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1guc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1guc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1guc RCSB], [http://www.ebi.ac.uk/pdbsum/1guc PDBsum]</span></td></tr>
+<table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The duplex (rGAGGUCUC)2 contains the motif [sequence: see text] which is unusually stable compared with other symmetric tandem GU mismatches and occurs in the P5 helix of the group I intron of Tetrahymena thermophila. The three-dimensional solution structure of (rGAGGUCUC)2 was determined using two-dimensional NMR and a simulated annealing protocol. The structure is remarkably similar to the A-DNA crystal structure of (dGGGGTCCC)2 [Kneale, G., Brown, T., &amp; Kennard, O. (1985) J. Mol. Biol. 186, 805-814] which contains the analogous motif [sequence: see text]. Incorporation of the [sequence: see text] motif has little effect on backbone torsion angles and helical parameters compared with standard A-form. The only significant departure from A-form is a slight overtwisting 5' of the G in the GU mismatch and a displacement of the mismatches toward the minor groove. Inspection of stacking patterns of this structure and comparison with symmetric tandem GT mismatches in A-DNA oligonucleotides from crystal structure data suggest that electrostatics are important in determining motif stability.
-{{STRUCTURE_1guc|  PDB=1guc  |  SCENE=  }}
+Investigation of the structural basis for thermodynamic stabilities of tandem GU mismatches: solution structure of (rGAGGUCUC)2 by two-dimensional NMR and simulated annealing.,McDowell JA, Turner DH Biochemistry. 1996 Nov 12;35(45):14077-89. PMID:8916893<ref>PMID:8916893</ref>
-===SOLUTION NMR STRUCTURE OF AN RNA WITH TANDEM, SYMMETRIC GU MISMATCHES, 30 STRUCTURES===
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
-{{ABSTRACT_PUBMED_8916893}}
+== References ==
+<references/>
-==About this Structure==
+__TOC__
-[[1guc]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GUC OCA].
+</StructureSection>
 [[Category: Mcdowell, J A.]]
 [[Category: Turner, D H.]]