3urg: Difference between revisions
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[[ | ==The crystal structure of Anabaena CcbP== | ||
<StructureSection load='3urg' size='340' side='right' caption='[[3urg]], [[Resolution|resolution]] 2.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[3urg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Nostoc_sp._pcc_7120 Nostoc sp. pcc 7120]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3URG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3URG FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MNB:5-MERCAPTO-2-NITRO-BENZOIC+ACID'>MNB</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2k2v|2k2v]], [[2p0p|2p0p]]</td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ccbP, alr1010 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=103690 Nostoc sp. PCC 7120])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3urg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3urg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3urg RCSB], [http://www.ebi.ac.uk/pdbsum/3urg PDBsum]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Obtaining crystals presented a bottleneck in the structural study of Anabaena cyanobacterial Ca(2+)-binding protein (CcbP). In this report, the promoting effect of Ellman's reagent [5,5'-dithiobis(2-nitrobenzoic acid); DTNB] on the crystallization of CcbP is described. CcbP contains one free cysteine. A quick and simple oxidation reaction with DTNB blocked the free cysteine in purified CcbP and generated a homogenous monomeric protein for crystallization. The crystal structure of DTNB-modified CcbP was determined by the single-wavelength anomalous diffraction method. Structure analysis indicated that DTNB modification facilitated crystallization of CcbP by inducing polar interactions in the crystal lattice. DTNB-mediated cysteine modification was demonstrated to have little effect on the overall structure and the Ca(2+) binding of CcbP. Thus, DTNB modification may provide a simple and general approach for protein modification to improve the success of crystallization screening. | |||
Ellman's reagent in promoting crystallization and structure determination of Anabaena CcbP.,Fan XX, Zhou YF, Liu X, Li LF, Su XD Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Nov 1;68(Pt 11):1409-14., doi: 10.1107/S1744309112034938. Epub 2012 Oct 30. PMID:23143261<ref>PMID:23143261</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
== References == | |||
<references/> | |||
== | __TOC__ | ||
</StructureSection> | |||
[[Category: Nostoc sp. pcc 7120]] | [[Category: Nostoc sp. pcc 7120]] | ||
[[Category: Fan, X X | [[Category: Fan, X X]] | ||
[[Category: Liu, X | [[Category: Liu, X]] | ||
[[Category: Su, X D | [[Category: Su, X D]] | ||
[[Category: Calcium binding protein]] | [[Category: Calcium binding protein]] | ||
[[Category: Metal binding protein]] | [[Category: Metal binding protein]] | ||
