1rld: Difference between revisions

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[[Image:1rld.jpg|left|200px]]<br /><applet load="1rld" size="350" color="white" frame="true" align="right" spinBox="true"
[[Image:1rld.jpg|left|200px]]
caption="1rld, resolution 2.5&Aring;" />
 
'''SOLID-STATE PHASE TRANSITION IN THE CRYSTAL STRUCTURE OF RIBULOSE 1,5-BIPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE'''<br />
{{Structure
|PDB= 1rld |SIZE=350|CAPTION= <scene name='initialview01'>1rld</scene>, resolution 2.5&Aring;
|SITE=
|LIGAND=
|ACTIVITY= [http://en.wikipedia.org/wiki/Ribulose-bisphosphate_carboxylase Ribulose-bisphosphate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39]
|GENE=
}}
 
'''SOLID-STATE PHASE TRANSITION IN THE CRYSTAL STRUCTURE OF RIBULOSE 1,5-BIPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE'''
 


==Overview==
==Overview==
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==About this Structure==
==About this Structure==
1RLD is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Active as [http://en.wikipedia.org/wiki/Ribulose-bisphosphate_carboxylase Ribulose-bisphosphate carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.39 4.1.1.39] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RLD OCA].  
1RLD is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RLD OCA].  


==Reference==
==Reference==
Solid-state phase transition in the crystal structure of ribulose 1,5-bisphosphate carboxylase/oxygenase., Zhang KY, Eisenberg D, Acta Crystallogr D Biol Crystallogr. 1994 May 1;50(Pt 3):258-62. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15299436 15299436]
Solid-state phase transition in the crystal structure of ribulose 1,5-bisphosphate carboxylase/oxygenase., Zhang KY, Eisenberg D, Acta Crystallogr D Biol Crystallogr. 1994 May 1;50(Pt 3):258-62. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15299436 15299436]
[[Category: Nicotiana tabacum]]
[[Category: Nicotiana tabacum]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: lyase(carbon-carbon)]]
[[Category: lyase(carbon-carbon)]]


''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:52:08 2008''
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Revision as of 11:53, 20 March 2008

File:1rld.jpg


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1rld, resolution 2.5Å
Activity: Ribulose-bisphosphate carboxylase, with EC number 4.1.1.39
Coordinates: save as pdb, mmCIF, xml



SOLID-STATE PHASE TRANSITION IN THE CRYSTAL STRUCTURE OF RIBULOSE 1,5-BIPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE


Overview

The crystal structure is described of ribulose 1,5-bisphosphate carboxylase/oxygenase in a new crystal form. This new form (form V) was obtained from a previously known crystal form (form III) through a solid-state phase transition. The solid-state phase transition was brought about by transferring the crystal from a high-salt low-pH mother liquor to a low-salt high-pH synthetic mother liquor. The interplay of electrostatic repulsion and osmotic pressure induced a unit-cell shrinkage of 24 A along the c axis and expansion of 4 A along the a and b axes. The space group also changed from I422 to I4. The new crystal form was shown to be more resistant to X-ray radiation damage, which suggests the effect of crystal stabilization by non-penetrating molecules. The structure of ribulose 1,5-bisphosphate carboxylase/oxygenase in the new crystal form is compared with that of the old crystal form.

About this Structure

1RLD is a Protein complex structure of sequences from Nicotiana tabacum. Full crystallographic information is available from OCA.

Reference

Solid-state phase transition in the crystal structure of ribulose 1,5-bisphosphate carboxylase/oxygenase., Zhang KY, Eisenberg D, Acta Crystallogr D Biol Crystallogr. 1994 May 1;50(Pt 3):258-62. PMID:15299436

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