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[[Image:1k6d.png|left|200px]]
==CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT==
<StructureSection load='1k6d' size='340' side='right' caption='[[1k6d]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1k6d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1K6D FirstGlance]. <br>
</td></tr><tr><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene><br>
<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Acetate_CoA-transferase Acetate CoA-transferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.8.3.8 2.8.3.8] </span></td></tr>
<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1k6d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1k6d OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1k6d RCSB], [http://www.ebi.ac.uk/pdbsum/1k6d PDBsum], [http://www.topsan.org/Proteins/MCSG/1k6d TOPSAN]</span></td></tr>
<table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/k6/1k6d_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The automation of protein structure determination is an essential component for high-throughput structural analysis in protein X-ray crystallography and is a key element in structural genomics. This highly challenging undertaking relies at present on the availability of high-quality native and derivatized protein crystals diffracting to high or moderate resolution, respectively. Obtaining such crystals often requires significant effort. The present study demonstrates that phases obtained at low resolution (&gt;3.0 A) from crystals of SeMet-labeled protein can be successfully used for automated structure determination. The crystal structure of acetate CoA-transferase alpha-subunit was solved using 3.4 A multi-wavelength anomalous dispersion data collected from a crystal containing SeMet-substituted protein and 1.9 A data collected from a native protein crystal.


{{STRUCTURE_1k6d|  PDB=1k6d  |  SCENE=  }}
Autotracing of Escherichia coli acetate CoA-transferase alpha-subunit structure using 3.4 A MAD and 1.9 A native data.,Korolev S, Koroleva O, Petterson K, Gu M, Collart F, Dementieva I, Joachimiak A Acta Crystallogr D Biol Crystallogr. 2002 Dec;58(Pt 12):2116-21. Epub 2002, Nov 23. PMID:12454473<ref>PMID:12454473</ref>


===CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT===
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
{{ABSTRACT_PUBMED_12454473}}
== References ==
 
<references/>
==About this Structure==
__TOC__
[[1k6d]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1K6D OCA].
</StructureSection>
 
==Reference==
<ref group="xtra">PMID:012454473</ref><ref group="xtra">PMID:015215460</ref><references group="xtra"/>
[[Category: Acetate CoA-transferase]]
[[Category: Acetate CoA-transferase]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]

Revision as of 14:12, 28 September 2014

CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT

1k6d, resolution 1.90Å

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