1s0u: Difference between revisions
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'''eIF2gamma apo''' | {{Structure | ||
|PDB= 1s0u |SIZE=350|CAPTION= <scene name='initialview01'>1s0u</scene>, resolution 2.4Å | |||
|SITE= | |||
|LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | |||
|ACTIVITY= | |||
|GENE= EIF2G, MJ1261 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2190 Methanocaldococcus jannaschii]) | |||
}} | |||
'''eIF2gamma apo''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1S0U is a [ | 1S0U is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S0U OCA]. | ||
==Reference== | ==Reference== | ||
X-ray structure of translation initiation factor eIF2gamma: implications for tRNA and eIF2alpha binding., Roll-Mecak A, Alone P, Cao C, Dever TE, Burley SK, J Biol Chem. 2004 Mar 12;279(11):10634-42. Epub 2003 Dec 19. PMID:[http:// | X-ray structure of translation initiation factor eIF2gamma: implications for tRNA and eIF2alpha binding., Roll-Mecak A, Alone P, Cao C, Dever TE, Burley SK, J Biol Chem. 2004 Mar 12;279(11):10634-42. Epub 2003 Dec 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14688270 14688270] | ||
[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: trna]] | [[Category: trna]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 13:58:58 2008'' | ||
Revision as of 11:59, 20 March 2008
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| 1s0u, resolution 2.4Å | |||||||||||||
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| Ligands: | ZN | ||||||||||||
| Gene: | EIF2G, MJ1261 (Methanocaldococcus jannaschii) | ||||||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
eIF2gamma apo
Overview
The x-ray structure of the gamma-subunit of the heterotrimeric translation initiation factor eIF2 has been determined to 2.4-A resolution. eIF2 is a GTPase that delivers the initiator Met-tRNA to the P site on the small ribosomal subunit during a rate-limiting initiation step in translation. The structure of eIF2gamma closely resembles that of EF1A.GTP, consisting of an N-terminal G domain followed by two beta-barrels arranged in a closed configuration with domain II packed against the G domain in the vicinity of the Switch regions. The G domain of eIF2gamma has an unusual zinc ribbon motif, not previously found in other GTPases. Structure-based site-directed mutagenesis was used to identify two adjacent features on the surface of eIF2gamma that bind the alpha-subunit and Met-tRNA(i)(Met), respectively. These structural, biochemical, and genetic results provide new insights into eIF2 ternary complex assembly.
About this Structure
1S0U is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
Reference
X-ray structure of translation initiation factor eIF2gamma: implications for tRNA and eIF2alpha binding., Roll-Mecak A, Alone P, Cao C, Dever TE, Burley SK, J Biol Chem. 2004 Mar 12;279(11):10634-42. Epub 2003 Dec 19. PMID:14688270
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