1s9s: Difference between revisions
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[[Image:1s9s.gif|left|200px]] | [[Image:1s9s.gif|left|200px]] | ||
'''SOLUTION STRUCTURE OF MLV PSI SITE''' | {{Structure | ||
|PDB= 1s9s |SIZE=350|CAPTION= <scene name='initialview01'>1s9s</scene> | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
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'''SOLUTION STRUCTURE OF MLV PSI SITE''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1S9S is a [ | 1S9S is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S9S OCA]. | ||
==Reference== | ==Reference== | ||
NMR structure of the 101-nucleotide core encapsidation signal of the Moloney murine leukemia virus., D'Souza V, Dey A, Habib D, Summers MF, J Mol Biol. 2004 Mar 19;337(2):427-42. PMID:[http:// | NMR structure of the 101-nucleotide core encapsidation signal of the Moloney murine leukemia virus., D'Souza V, Dey A, Habib D, Summers MF, J Mol Biol. 2004 Mar 19;337(2):427-42. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15003457 15003457] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Dey, A.]] | [[Category: Dey, A.]] | ||
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[[Category: u-u mismatch]] | [[Category: u-u mismatch]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:02:11 2008'' | ||
Revision as of 12:02, 20 March 2008
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SOLUTION STRUCTURE OF MLV PSI SITE
Overview
The full length, positive-strand genome of the Moloney Murine Leukemia Virus contains a "core encapsidation signal" that is essential for efficient genome packaging during virus assembly. We have determined the structure of a 101-nucleotide RNA that contains this signal (called mPsi) using a novel isotope-edited NMR approach. The method is robust and should be generally applicable to larger RNAs. mPsi folds into three stem loops, two of which (SL-C and SL-D) co-stack to form an extended helix. The third stem loop (SL-B) is connected to SL-C by a flexible, four-nucleotide linker. The structure contains five mismatched base-pairs, an unusual C.CG base-triple platform, and a novel "A-minor K-turn," in which unpaired adenosine bases A340 and A341 of a GGAA bulge pack in the minor groove of a proximal stem, and a bulged distal uridine (U319) forms a hydrogen bond with the phosphodiester of A341. Phylogenetic analyses indicate that these essential structural elements are conserved among the murine C-type retroviruses.
About this Structure
1S9S is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
NMR structure of the 101-nucleotide core encapsidation signal of the Moloney murine leukemia virus., D'Souza V, Dey A, Habib D, Summers MF, J Mol Biol. 2004 Mar 19;337(2):427-42. PMID:15003457
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