4gmk: Difference between revisions
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[[ | ==Crystal Structure of Ribose 5-Phosphate Isomerase from the Probiotic Bacterium Lactobacillus salivarius UCC118== | ||
<StructureSection load='4gmk' size='340' side='right' caption='[[4gmk]], [[Resolution|resolution]] 1.72Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4gmk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lactobacillus_salivarius_ucc118 Lactobacillus salivarius ucc118]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4GMK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4GMK FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rpiA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=362948 Lactobacillus salivarius UCC118])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribose-5-phosphate_isomerase Ribose-5-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.6 5.3.1.6] </span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4gmk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4gmk OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4gmk RCSB], [http://www.ebi.ac.uk/pdbsum/4gmk PDBsum]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/Q1WR87_LACS1 Q1WR87_LACS1]] Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.[HAMAP-Rule:MF_00170][SAAS:SAAS00020827] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The structure of ribose 5-phosphate isomerase from the probiotic bacterium Lactobacillus salivarius UCC188 has been determined at 1.72 A resolution. The structure was solved by molecular replacement, which identified the functional homodimer in the asymmetric unit. Despite only showing 57% sequence identity to its closest homologue, the structure adopted the typical alpha and beta D-ribose 5-phosphate isomerase fold. Comparison to other related structures revealed high homology in the active site, allowing a model of the substrate-bound protein to be proposed. The determination of the structure was expedited by the use of in situ crystallization-plate screening on beamline I04-1 at Diamond Light Source to identify well diffracting protein crystals prior to routine cryocrystallography. | |||
Structure of ribose 5-phosphate isomerase from the probiotic bacterium Lactobacillus salivarius UCC118.,Lobley CM, Aller P, Douangamath A, Reddivari Y, Bumann M, Bird LE, Nettleship JE, Brandao-Neto J, Owens RJ, O'Toole PW, Walsh MA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1427-33., doi: 10.1107/S174430911204273X. Epub 2012 Nov 14. PMID:23192019<ref>PMID:23192019</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
==See Also== | |||
*[[Ribose-5-phosphate isomerase|Ribose-5-phosphate isomerase]] | |||
== | == References == | ||
[[ | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Lactobacillus salivarius ucc118]] | [[Category: Lactobacillus salivarius ucc118]] | ||
[[Category: Ribose-5-phosphate isomerase]] | [[Category: Ribose-5-phosphate isomerase]] | ||
[[Category: Aller, P | [[Category: Aller, P]] | ||
[[Category: Bird, L E | [[Category: Bird, L E]] | ||
[[Category: Brandao-Neto, J | [[Category: Brandao-Neto, J]] | ||
[[Category: Bumann, M | [[Category: Bumann, M]] | ||
[[Category: Douangamath, A | [[Category: Douangamath, A]] | ||
[[Category: Lobley, C M.C | [[Category: Lobley, C M.C]] | ||
[[Category: Owens, R J | [[Category: Owens, R J]] | ||
[[Category: Reddivari, Y | [[Category: Reddivari, Y]] | ||
[[Category: Toole, P W.O | [[Category: Toole, P W.O]] | ||
[[Category: Walsh, M A | [[Category: Walsh, M A]] | ||
[[Category: D-ribose-5-phosphate isomerase family]] | [[Category: D-ribose-5-phosphate isomerase family]] | ||
[[Category: Isomerase]] | [[Category: Isomerase]] | ||
[[Category: Ribose 5-phosphate isomerisation]] | [[Category: Ribose 5-phosphate isomerisation]] | ||
Revision as of 14:07, 4 January 2015
Crystal Structure of Ribose 5-Phosphate Isomerase from the Probiotic Bacterium Lactobacillus salivarius UCC118
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