1sip: Difference between revisions
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[[Image:1sip.gif|left|200px]] | [[Image:1sip.gif|left|200px]] | ||
'''ALTERNATIVE NATIVE FLAP CONFORMATION REVEALED BY 2.3 ANGSTROMS RESOLUTION STRUCTURE OF SIV PROTEINASE''' | {{Structure | ||
|PDB= 1sip |SIZE=350|CAPTION= <scene name='initialview01'>1sip</scene>, resolution 2.3Å | |||
|SITE= | |||
|LIGAND= | |||
|ACTIVITY= | |||
|GENE= | |||
}} | |||
'''ALTERNATIVE NATIVE FLAP CONFORMATION REVEALED BY 2.3 ANGSTROMS RESOLUTION STRUCTURE OF SIV PROTEINASE''' | |||
==Overview== | ==Overview== | ||
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==About this Structure== | ==About this Structure== | ||
1SIP is a [ | 1SIP is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Simian_immunodeficiency_virus Simian immunodeficiency virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SIP OCA]. | ||
==Reference== | ==Reference== | ||
Alternative native flap conformation revealed by 2.3 A resolution structure of SIV proteinase., Wilderspin AF, Sugrue RJ, J Mol Biol. 1994 May 27;239(1):97-103. PMID:[http:// | Alternative native flap conformation revealed by 2.3 A resolution structure of SIV proteinase., Wilderspin AF, Sugrue RJ, J Mol Biol. 1994 May 27;239(1):97-103. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8196050 8196050] | ||
[[Category: Simian immunodeficiency virus]] | [[Category: Simian immunodeficiency virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: hydrolase(acid proteinase)]] | [[Category: hydrolase(acid proteinase)]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 14:05:25 2008'' | ||
Revision as of 12:05, 20 March 2008
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| 1sip, resolution 2.3Å | |||||||||||||
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| Coordinates: | save as pdb, mmCIF, xml | ||||||||||||
ALTERNATIVE NATIVE FLAP CONFORMATION REVEALED BY 2.3 ANGSTROMS RESOLUTION STRUCTURE OF SIV PROTEINASE
Overview
A large conformational change is observed between HIV-1 proteinase in the ligand-free state and in complexes with transition-state inhibitors. Crystal structures of this enzyme have either the flaps open for the native or ligand-free enzyme or the flaps closed for peptidomimetic ligand-bound enzyme. We describe the structure of native recombinant SIV proteinase which like other retroviral proteinases crystallizes as a perfect 2-fold symmetric dimer but in a different crystal packing arrangement. In contrast to HIV-1 PR we show that SIV proteinase in the ligand-free state adopts the closed flaps conformation, demonstrating that ligand binding is not a prerequisite for the closed flaps conformation. The catalytic water was clearly observed between the two aspartates which were not perfectly co-planar, and in this structure the active site cleft is more restricted than for either inhibitor bound or ligand-free HIV-1 proteinase. Accommodation of two bulkier side-chains in the simian enzyme core has resulted in a more exposed N terminus than for HIV-1 PR which we predict could enhance autocatalytic cleavage at the N terminus.
About this Structure
1SIP is a Single protein structure of sequence from Simian immunodeficiency virus. Full crystallographic information is available from OCA.
Reference
Alternative native flap conformation revealed by 2.3 A resolution structure of SIV proteinase., Wilderspin AF, Sugrue RJ, J Mol Biol. 1994 May 27;239(1):97-103. PMID:8196050
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